Fluorescein-12-dUTP

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Fluorescein-12-dUTP

Fluorescein-12-dUTP

Catalog Number A07-0022
Category RNA/DNA Labeling
Molecular Formula C39H41N4O21P3(free acid)
Molecular Weight 994.69 (free acid)
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Product Introduction

Fluorescein-12-dUTP is a pivotal instrument employed in the realm of biomedical research, serving as an impeccably precise enquirer for the intricate realm of DNA fragmentation and the discernment of apoptosis. It is extensively harnessed as a propitious catalyst within the domain of molecular biology methodologies, including fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR)y.

  • Chemical Information

  • Product Specification

  • Computed Properties

Synonyms Fluorescein-X-5-Aminoallyl-dUTP; 5/6-Fluorescein-X-5-aminoallyl-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
Purity ≥ 95 % by HPLC
IUPAC Name [[5-[5-[3-[6-[(3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carbonyl)amino]hexanoylamino]prop-1-enyl]-2,4-dioxo-1,3-diazinan-1-yl]-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
Canonical SMILES C1C(C(OC1N2CC(C(=O)NC2=O)C=CCNC(=O)CCCCCNC(=O)C3=CC4=C(C=C3)C5(C6=C(C=C(C=C6)O)OC7=C5C=CC(=C7)O)OC4=O)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O
InChI InChI=1S/C39H43N4O21P3/c44-23-8-11-27-30(16-23)60-31-17-24(45)9-12-28(31)39(27)26-10-7-21(15-25(26)37(50)62-39)35(48)41-13-3-1-2-6-33(47)40-14-4-5-22-19-43(38(51)42-36(22)49)34-18-29(46)32(61-34)20-59-66(55,56)64-67(57,58)63-65(52,53)54/h4-5,7-12,15-17,22,29,32,34,44-46H,1-3,6,13-14,18-20H2,(H,40,47)(H,41,48)(H,55,56)(H,57,58)(H,42,49,51)(H2,52,53,54)
InChIKey QTGRZULTMFHDJZ-UHFFFAOYSA-N
pH 7.5 ±0.5
Appearance yellow-orange solution in water
Spectroscopic Properties λexc 492 nm, λem 517 nm, ε 83.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Storage store at-20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible.
XLogP3 -2
Hydrogen Bond Donor Count 10
Hydrogen Bond Acceptor Count 21
Rotatable Bond Count 18
Exact Mass 996.16326539 g/mol
Monoisotopic Mass 996.16326539 g/mol
Topological Polar Surface Area 373Ų
Heavy Atom Count 67
Formal Charge 0
Complexity 2020
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 4
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 1
Covalently-Bonded Unit Count 1
Compound Is Canonicalized Yes

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