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Fluorescein-12-dUTP
| Catalog Number | A07-0022 |
| Category | RNA/DNA Labeling |
| Molecular Formula | C39H41N4O21P3(free acid) |
| Molecular Weight | 994.69 (free acid) |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A07-0022 | -- | $-- |
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Product Introduction
Fluorescein-12-dUTP is a pivotal instrument employed in the realm of biomedical research, serving as an impeccably precise enquirer for the intricate realm of DNA fragmentation and the discernment of apoptosis. It is extensively harnessed as a propitious catalyst within the domain of molecular biology methodologies, including fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR)y.
Chemical Information |
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|---|---|
| Synonyms | Fluorescein-X-5-Aminoallyl-dUTP; 5/6-Fluorescein-X-5-aminoallyl-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt |
| Purity | ≥ 95 % by HPLC |
| IUPAC Name | [[5-[5-[3-[6-[(3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carbonyl)amino]hexanoylamino]prop-1-enyl]-2,4-dioxo-1,3-diazinan-1-yl]-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate |
| Canonical SMILES | C1C(C(OC1N2CC(C(=O)NC2=O)C=CCNC(=O)CCCCCNC(=O)C3=CC4=C(C=C3)C5(C6=C(C=C(C=C6)O)OC7=C5C=CC(=C7)O)OC4=O)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O |
| InChI | InChI=1S/C39H43N4O21P3/c44-23-8-11-27-30(16-23)60-31-17-24(45)9-12-28(31)39(27)26-10-7-21(15-25(26)37(50)62-39)35(48)41-13-3-1-2-6-33(47)40-14-4-5-22-19-43(38(51)42-36(22)49)34-18-29(46)32(61-34)20-59-66(55,56)64-67(57,58)63-65(52,53)54/h4-5,7-12,15-17,22,29,32,34,44-46H,1-3,6,13-14,18-20H2,(H,40,47)(H,41,48)(H,55,56)(H,57,58)(H,42,49,51)(H2,52,53,54) |
| InChI Key | QTGRZULTMFHDJZ-UHFFFAOYSA-N |
| pH | 7.5 ±0.5 |
| Appearance | yellow-orange solution in water |
- Product Specification
| Spectroscopic Properties | λexc 492 nm, λem 517 nm, ε 83.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) |
| Storage | store at-20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible. |
Computed Properties | |
|---|---|
| XLogP3 | -2 |
| Hydrogen Bond Donor Count | 10 |
| Hydrogen Bond Acceptor Count | 21 |
| Rotatable Bond Count | 18 |
| Exact Mass | 996.16326539 g/mol |
| Monoisotopic Mass | 996.16326539 g/mol |
| Topological Polar Surface Area | 373Ų |
| Heavy Atom Count | 67 |
| Formal Charge | 0 |
| Complexity | 2020 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 4 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 1 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
Applications of Fluorescent Probes & Dyes
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