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AMCA-6-dUTP
| Catelog Number | A07-0016 |
| Category | RNA/DNA Labeling |
| Molecular Formula | C30H40N5O18P3 (free acid) |
| Molecular Weight | 851.58 (free acid) |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A07-0016 | -- | $-- |
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Product Introduction
AMCA-6-dUTP is a fluorescent probe commonly used in the biomedical industry to label DNA during various molecular biology applications. It can be incorporated into DNA using DNA polymerase and serving as a valuable tool for visualizing and studying DNA replication, repair and recombination.
Chemical Information |
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|---|---|
| Synonyms | Aminoallyl-dUTP - AMCA; Aminomethylcoumarin-6-dUTP; Aminomethylcoumarin-5-aminoallyl-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt |
| Purity | ≥ 95% by HPLC |
| IUPAC Name | [[(2R,3S,5R)-5-[5-[(E)-3-[6-[[2-(7-amino-4-methyl-2-oxochromen-3-yl)acetyl]amino]hexanoylamino]prop-1-enyl]-2,4-dioxopyrimidin-1-yl]-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate |
| Canonical SMILES | CC1=C(C(=O)OC2=C1C=CC(=C2)N)CC(=O)NCCCCCC(=O)NCC=CC3=CN(C(=O)NC3=O)C4CC(C(O4)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O |
| InChI | InChI=1S/C30H40N5O18P3/c1-17-20-9-8-19(31)12-23(20)51-29(40)21(17)13-26(38)33-10-4-2-3-7-25(37)32-11-5-6-18-15-35(30(41)34-28(18)39)27-14-22(36)24(50-27)16-49-55(45,46)53-56(47,48)52-54(42,43)44/h5-6,8-9,12,15,22,24,27,36H,2-4,7,10-11,13-14,16,31H2,1H3,(H,32,37)(H,33,38)(H,45,46)(H,47,48)(H,34,39,41)(H2,42,43,44)/b6-5+/t22-,24+,27+/m0/s1 |
| InChI Key | JOYRMWPIHHXOPJ-CFQWBBOESA-N |
| pH | 7.5 ±0.5 |
| Appearance | sterile clear aqueous solution, in 10 mM Tris-HCl |
Product Specification |
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|---|---|
| Spectroscopic Properties | λexc 350 nm, λem 450 nm, ε 19.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) |
| Storage | store at-20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible. |
Computed Properties | |
|---|---|
| XLogP3 | -4.3 |
| Hydrogen Bond Donor Count | 9 |
| Hydrogen Bond Acceptor Count | 19 |
| Rotatable Bond Count | 19 |
| Exact Mass | 851.15812044 g/mol |
| Monoisotopic Mass | 851.15812044 g/mol |
| Topological Polar Surface Area | 349Ų |
| Heavy Atom Count | 56 |
| Formal Charge | 0 |
| Complexity | 1770 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 3 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 1 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
Applications of Fluorescent Probes & Dyes
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