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DEAC-dUTP
| Catalog Number | A07-0017 |
| Category | RNA/DNA Labeling |
| Molecular Formula | C26H33N4O17P3 (free acid) |
| Molecular Weight | 766.48 (free acid) |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
DEAC-dUTP is a remarkable nucleotide, finding application in the field of molecular biology studies. It contributes indispensably to endeavors encompassing DNA sequencing, PCR techniques and DNA labeling.
Chemical Information
Product Specification
Application
| Synonyms | Aminoallyl-dUTP - DEAC; Diethylaminocoumarin-5-dUTP; Diethylaminocoumarin-5-aminoallyl-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt |
| Purity | ≥ 95% by HPLC |
| pH | 7.5 ±0.5 |
| Appearance | sterile yellow aqueous solution, in 10 mM Tris-HCl |
| Spectroscopic Properties | λexc 426 nm, λem 480 nm, ε 57.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) |
| Storage | store at-20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible. |
DEAC-dUTP is a fluorescent nucleotide analog widely used in bioscience research. Here are some key applications of DEAC-dUTP:
Fluorescent Labeling: DEAC-dUTP is utilized for labeling nucleic acids in a variety of fluorescence-based assays. Researchers incorporate DEAC-dUTP into DNA or RNA using polymerases during synthesis. This enables the detection and visualization of nucleic acids in techniques such as fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR).
DNA Sequencing: DEAC-dUTP can be used in next-generation sequencing (NGS) platforms due to its fluorescent properties. By incorporating DEAC-dUTP into DNA strands, high-throughput sequencing machines can accurately read sequence data based on the specific fluorescence emission. This facilitates the identification of genetic variations and mutations in genomic studies.
Live Cell Imaging: DEAC-dUTP is employed in live cell imaging studies to monitor DNA replication and repair processes in real-time. When introduced into living cells, DEAC-dUTP integrates into newly synthesized DNA during replication. The fluorescent signal allows researchers to track and analyze dynamic changes in DNA metabolism within the cellular context.
Flow Cytometry: DEAC-dUTP is also used in flow cytometry for the analysis of cell proliferation. Cells are incubated with DEAC-dUTP, which gets incorporated into newly synthesized DNA. The fluorescence intensity is then measured, providing insights into the proliferation rate and cell cycle status of different cell populations.
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