SYBR Gold | CAS 213019-16-0

SYBR Gold is a high-sensitivity nucleic acid gel stain and fluorescence dye designed for DNA and RNA visualization with strong fluorescence enhancement upon binding to nucleic acids. Its bright green emission and widely used staining workflow make it a standard reagent for electrophoresis readouts, downstream sizing/quantification, and nucleic-acid quality control in molecular biology and analytical chemistry laboratories.

1. DNA Gel Electrophoresis Staining

SYBR Gold is routinely used by molecular biology labs to visualize DNA after agarose or polyacrylamide gel electrophoresis, enabling convenient band detection for plasmid analysis, restriction digest verification, and PCR product assessment. Researchers typically integrate the dye into post-staining workflows to generate high-contrast gel images using common fluorescence gel documentation systems, supporting routine fragment sizing and qualitative comparisons across samples.

2. RNA Gel Visualization And QC

SYBR Gold is also applied for RNA analysis where fluorescent nucleic-acid staining is needed to confirm RNA integrity and approximate abundance following electrophoresis. Molecular biology and RNA-focused workflows often rely on SYBR Gold staining to monitor sample quality across extraction batches and to support downstream experiments such as RT-PCR and library preparation, using the dye's nucleic-acid binding response to produce clear fluorescence readouts.

3. Fluorescence-Based Nucleic Acid Quantification

SYBR Gold is used in fluorescence assays for nucleic-acid quantification in research settings, including plate-based workflows where nucleic-acid standards and unknowns are compared by fluorescence intensity. Biochemistry and genomics groups commonly employ the dye for normalization and concentration checks prior to sensitive downstream steps, leveraging its nucleic-acid-dependent fluorescence signal to convert sample nucleic-acid content into measurable optical readouts.

4. qPCR And Nucleic Acid Assay Development

SYBR Gold is frequently incorporated into fluorescence-based nucleic acid assay development, particularly where intercalating dyes are used as readout reagents for amplification monitoring. Assay developers use SYBR Gold in experimental optimization of primer sets and reaction conditions, and in workflows that require real-time fluorescence tracking of nucleic-acid accumulation using standard qPCR instrumentation and compatible dye detection channels.