Sulfo-Cyanine5.5 dUTP

Sulfo-Cyanine5.5 dUTP is a fluorescently labeled deoxyuridine triphosphate designed for nucleic acid incorporation workflows, enabling direct fluorescent tagging of DNA during enzymatic synthesis. As a sulfonated cyanine dye nucleotide analog, it supports aqueous labeling while providing a bright far-red emission signal that is commonly read on fluorescence microscopes and fluorescence-capable plate readers. Researchers use it to introduce a Cy5.5 fluorophore into probes, templates, and labeled nucleic acid standards for imaging and fluorescence-based assays.

1. Fluorescent DNA Probe Labeling

Sulfo-Cyanine5.5 dUTP is used to generate fluorescent DNA probes by enzymatic incorporation into oligonucleotides, PCR products, or nick-translation style labeling workflows. Molecular biology and assay development teams rely on the far-red cyanine emission to visualize labeled DNA targets with reduced spectral interference from common green fluorophores, supporting probe-based hybridization readouts in microscopy and fluorescence detection formats. The sulfonated dye functionality helps maintain labeling performance in aqueous buffers used for hybridization and washing steps.

2. Fluorescence In Situ Hybridization

Sulfo-Cyanine5.5 dUTP is frequently selected for fluorescence in situ hybridization (FISH) experiments where DNA probes are prepared with far-red fluorescence for cellular imaging. Cytogenetics, genome biology, and cell imaging laboratories use Cy5.5-labeled nucleic acids to localize specific DNA sequences in fixed samples and to multiplex with other fluorophores when spectral separation is required. Because the dye is incorporated into the probe during nucleic acid synthesis, the resulting probe can be used directly in hybridization workflows without requiring post-labeling dye conjugation steps.

3. qPCR and Nucleic Acid Amplification Imaging

Sulfo-Cyanine5.5 dUTP is applied in fluorescence-based nucleic acid amplification and downstream imaging workflows where incorporation of labeled nucleotides enables direct fluorescent tracking of DNA synthesis products. Molecular diagnostics research groups and nucleic acid assay developers use Cy5.5-labeled amplicons as fluorescent standards, visualization reagents, or calibration materials for fluorescence readouts that detect amplified DNA. The water-soluble sulfonated dye character supports practical handling in typical amplification and buffer systems used for nucleic acid experiments.

4. Labeled DNA Standards And Controls

Sulfo-Cyanine5.5 dUTP supports preparation of fluorescent DNA standards and assay controls used in nucleic acid analysis workflows. Researchers in genomics and molecular imaging use Cy5.5-labeled DNA to validate hybridization performance, check instrument alignment, and provide reference signals for fluorescence quantification across experiments. By incorporating the dye into DNA during synthesis, laboratories can generate reproducible fluorescent materials for method development, optimization, and routine quality checks in fluorescence-based nucleic acid assays.