FAM-11-dCTP, 6-isomer

Product Introduction

6-fluorescein (FAM) derivative of deoxycytidine triphosphate (dCTP).,FAM 6-isomer is a common fluorophore used for labeling biomolecules; FAM has a high quantum yield and bright green emission.,FAM-11-dCTP can be used for enzymatic DNA labeling via Nick-translation, 3’-end labeling, random primed labeling, PCR, or cDNA synthesis. FAM-11-dCTP is incorporated into the growing DNA chain by Taq-polymerase, DNA-polymerase I, Phi 29 polymerase, Klenow fragment, reverse transcriptase, or terminal transferase.,The linker length (11 carbon atoms) is optimal for the efficient incorporation of fluorescently-labeled dCTP into the growing DNA chain.,Labeled DNA can be used for FISH experiments, microarray gene profiling, Southern blot, and Northern blot.

Chemical Information
Purity	HPLC-MS (95%), testing in enzymatic reaction
IUPAC Name	trilithium;[[[(2R,3S,5R)-5-[6-amino-5-[3-[6-[[3-carboxy-4-(3-hydroxy-6-oxoxanthen-9-yl)benzoyl]amino]hexanoylamino]prop-1-ynyl]-2-oxo-1,6-dihydropyrimidin-3-yl]-3-hydroxyoxolan-2-yl]methoxy-oxidophosphoryl]oxy-oxidophosphoryl] hydrogen phosphate
Canonical SMILES	[Li+].[Li+].[Li+].C1C(C(OC1N2C=C(C(NC2=O)N)C#CCNC(=O)CCCCCNC(=O)C3=CC(=C(C=C3)C4=C5C=CC(=O)C=C5OC6=C4C=CC(=C6)O)C(=O)O)COP(=O)([O-])OP(=O)([O-])OP(=O)(O)[O-])O
InChI	InChI=1S/C39H42N5O20P3.3Li/c40-36-22(19-44(39(52)43-36)34-18-29(47)32(62-34)20-60-66(56,57)64-67(58,59)63-65(53,54)55)5-4-14-41-33(48)6-2-1-3-13-42-37(49)21-7-10-25(28(15-21)38(50)51)35-26-11-8-23(45)16-30(26)61-31-17-24(46)9-12-27(31)35;;;/h7-12,15-17,19,29,32,34,36,45,47H,1-3,6,13-14,18,20,40H2,(H,41,48)(H,42,49)(H,43,52)(H,50,51)(H,56,57)(H,58,59)(H2,53,54,55);;;/q;3*+1/p-3/t29-,32+,34+,36?;;;/m0.../s1
InChI Key	WXKYUDILHYOXAO-BPWIAOORSA-K
Solubility	Good in water
Appearance	Yellow/Orange solid

Product Specification
CF260	0.20
CF280	0.17
Fluorescence Quantum Yield	0.93
Excitation	490
Emission	513
Storage	Storage: 12 months after receival at -20 °C in the Dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles.

Computed Properties
Hydrogen Bond Donor Count	8
Hydrogen Bond Acceptor Count	21
Rotatable Bond Count	19
Exact Mass	1011.1881349 g/mol
Monoisotopic Mass	1011.1881349 g/mol
Topological Polar Surface Area	398Ų
Heavy Atom Count	70
Formal Charge	0
Complexity	2280
Isotope Atom Count	0
Defined Atom Stereocenter Count	3
Undefined Atom Stereocenter Count	1
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
Covalently-Bonded Unit Count	4
Compound Is Canonicalized	Yes