DusQ1 CPG 500

Product Introduction

This modified support with a pore size of 500 Å is intended for the synthesis of oligonucleotides of up to 50 bases in length modified with non-fluorescent DusQ1 quencher at the 3’ end.,DusQ1 Dark quencher exhibits the strongest absorption within the range of 480 to 580 nm; its absorption maximum is at 534 nm. It can be used for combined quenching (a combination of static and dynamic quenching) of many fluorophores, including Biosearch Blue™, Marina Blue™, Edans, Bothell Blue, FAM™, JOE™, VIC™, R6G, HEX™, TET™, CAL Fluor™ Gold 540, and Yakima Yellow™. It can be used for the synthesis of hybridization probes such as TaqMan, Molecular Beacon, Scorpion.,Coupling: Standard conditions identical to normal nucleobases.,Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.

Chemical Information
Purity	NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis.
Appearance	Purple beads

Product Specification
CF260	0.17
CF280	0.10
Excitation	522
Storage	Storage: 24 months after receival at -20 °C in the Dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.