dsSafe Gel Staining Solution 10,000×

Name: dsSafe Gel Staining Solution 10,000×
Brand: BOC Sciences
Rating: 5 (1 reviews)

Catalog Number	A19-0066
Category	DNA Stains

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Product Introduction

The dsSafe dye is a fluorescent dye for nucleic acid staining in agarose and polyacrylamide gels. dsSafe is the best and safer alternative to ethidium bromide (EtBr); it was shown to be less toxic, non-mutagenic, and non-carcinogenic.,dsSafe allows nucleic acid visualization with sensitivity similar to that of ethidium bromide. Dye excitation can be performed with both conventional UV transilluminators and blue light transilluminators, with the latter ones being safer for the eyes and nucleic acid sample. When blue light is used for dsSafe excitation, background fluorescence is lower than in the case of ethidium bromide excitation with UV light. Excised DNA fragments for subsequent cloning are not exposed to damaging UV radiation, thus increasing cloning efficacy and reducing the frequency of adverse mutations caused by UV.,The spectrum of the DNA-dye complex has two excitation maxima [in the UV (280 nm) and blue ranges (502 nm)] and one emission maximum in the green range (~530 nm). Available as 10,000× concentrate in DMSO. To stain the gel, add the dye to melted agarose solution* or stain the gel after gel electrophoresis in TAE/TBE buffer for 10-20 minutes.,For nucleic acid gel staining, we also offer dsGreen Gel Staining Solution with higher sensitivity to DNA . However, in contrast with dsSafe, gel staining with dsGreen Gel Staining Solution is recommended only after the completion of gel electrophoresis.,*Similarly to ethidium bromide, dsSafe when added in melted agarose solution can slightly reduce the mobility of nucleic acid sample in the gel.

Chemical Information
Product Specification
Application

Chemical Information

Appearance

Orange solution

Product Specification

Storage

Storage: 24 months after receival at -20 °C in the Dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Application

dsSafe Gel Staining Solution 10,000× is a nucleic-acid gel staining reagent designed for post-run visualization of double-stranded DNA in electrophoresis workflows. The concentrated formulation is intended for convenient dilution and robust staining of agarose gels, supporting routine DNA analysis in molecular biology and genomics research. Researchers use it to monitor PCR products, restriction digests, and DNA fragment sizing with standard UV or blue-light imaging setups depending on the instrument configuration.

1. Agarose Gel DNA Visualization

dsSafe Gel Staining Solution 10,000× is used by molecular biology teams to stain agarose gels after electrophoresis for visualization of double-stranded DNA bands. In routine workflows such as PCR screening, cloning verification, and restriction digest analysis, the reagent provides a practical way to confirm product presence and assess fragment sizes using gel documentation systems. The high-concentration format supports consistent staining across many runs when diluted according to laboratory protocols.

2. PCR Product Screening

dsSafe Gel Staining Solution 10,000× supports high-throughput PCR screening in research labs by enabling rapid gel-based readout of amplification success. Teams commonly stain gels to distinguish specific amplicons from nonspecific bands and primer-dimer artifacts, then use the band patterns to guide downstream steps such as gel extraction or colony screening. This makes the reagent a convenient component of standard PCR-to-analysis pipelines in molecular diagnostics research environments and academic laboratories.

3. Restriction Digest and Cloning Checks

dsSafe Gel Staining Solution 10,000× is applied for analyzing restriction enzyme digests and ligation/cloning constructs by visualizing dsDNA fragments resolved on agarose gels. Researchers rely on the staining to verify expected banding patterns, confirm successful digestion, and estimate fragment sizes before proceeding to sequencing or further assembly. The concentrated formulation is particularly useful in cloning workflows where gels are run frequently and consistent band visualization is required for decision-making.

4. DNA Fragment Sizing

dsSafe Gel Staining Solution 10,000× is used to support DNA sizing and fragment comparison by staining electrophoresed dsDNA alongside DNA size standards. In genomics and library-prep research, gel images generated after staining help researchers evaluate whether fragments fall within expected size ranges prior to purification and downstream processing. The reagent's role in generating clear gel documentation makes it a common choice for iterative optimization of sample preparation and electrophoresis conditions.