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Product Introduction
dsGreen is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels. A qPCR grade reagent is also available.,Unlike ethidium bromide, dsGreen is highly selective towards double stranded DNA, much less harmful, and offers better sensitivity.
Chemical Information
Product Specification
Application
Chemical Information
| Purity | NMR 1H, HPLC-MS (95%), functional testing |
| Appearance | Orange solution |
Product Specification
| Fluorescence Quantum Yield | 0.8 |
| Excitation | 454 |
| Emission | 524 |
| Storage | Storage: 24 months after receival at -20 °C in the Dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
Application
dsGreen Gel Staining Solution, 10000× is a concentrated fluorescent gel stain designed for visualization of nucleic acids and proteins following electrophoresis. In routine electrophoresis workflows, dsGreen provides a convenient staining step that enables post-run imaging on standard fluorescence gel documentation systems. The high concentration format supports efficient use in gel-staining protocols commonly used in molecular biology, genomics, and protein analysis workflows.
1. Agarose Gel DNA Visualization
dsGreen Gel Staining Solution, 10000× is used to stain DNA in agarose gel electrophoresis workflows for routine fragment sizing, band pattern assessment, and gel-based quality checks of PCR products and restriction digests. Researchers and core facilities typically incorporate the stain into post-electrophoresis staining and then image the gels using a fluorescence gel doc system, enabling clear visualization of nucleic acid bands without the need for radioactive labeling. The concentrated formulation is particularly convenient for laboratories that run gels frequently and want consistent staining across many experiments.
2. SDS-PAGE Protein Band Imaging
dsGreen Gel Staining Solution, 10000× is also applied for fluorescent visualization of protein bands after SDS-PAGE, supporting electrophoresis-based protein profiling in biochemistry and molecular biology labs. Investigators use the stain to assess separation quality, compare relative band patterns across samples, and document gel results as part of downstream workflows such as cloning verification, expression screening, or sample normalization prior to blotting or mass spectrometry. Because the workflow is compatible with fluorescence gel imaging, it fits naturally into standard gel documentation pipelines used in research and industrial R&D environments.
3. High-Throughput Gel Documentation
dsGreen Gel Staining Solution, 10000× is frequently selected for throughput-oriented electrophoresis workflows where many gels must be stained and imaged consistently. The 10000× concentration format supports economical reagent usage, which is valuable for screening experiments, routine method development, and training or QC-style gel runs in shared laboratory settings. Teams typically rely on the stain to generate reproducible fluorescence readouts on gel documentation instruments, facilitating efficient documentation and comparison of gel runs across projects.
4. Educational And Method Development Gels
dsGreen Gel Staining Solution, 10000× is well suited for teaching labs and method development studies that require straightforward, non-radioactive gel visualization for student training and protocol optimization. In these settings, researchers can quickly incorporate the stain into electrophoresis practice to visualize nucleic acid or protein separation outcomes and verify that sample loading, running conditions, and gel handling steps are producing expected band patterns. The concentrated supply format also makes it practical for repeated classroom or internal training use where reagent cost and ease of workflow are important.
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