Cyanine5 phosphoramidite

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Cyanine5 phosphoramidite

Cyanine5 phosphoramidite

Catalog Number A19-0085
Category DNA Stains
Molecular Formula C47H67N5ClO3P
Molecular Weight 816.49
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Product Introduction

Cyanine5 is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines.,This reactive derivative is useful for the synthesis of qPCR probes having Cyanine5 at 5'-end, the most typical location of fluorophore. This is a terminating, non-nucleoside reagent.,Amidite group in this reagent is bound to a secondary oxygen. This provides extra stability against Arbuzov rearrangement-a reaction that leads to amidite deterioration in solution in oligonucleotide synthesizer. This molecular design increases stability of the reagent, maintaining efficient coupling over longer time.

  • Chemical Information

  • Product Specification

  • Computed Properties

Purity NMR 1H, 31P, HPLC-MS (80%)
IUPAC Name N-[4-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxycyclohexyl]-6-[3,3-dimethyl-2-[5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indol-1-yl]hexanamide;chloride
Canonical SMILES CC(C)N(C(C)C)P(OCCC#N)OC1CCC(CC1)NC(=O)CCCCCN2C3=CC=CC=C3C(C2=CC=CC=CC4=[N+](C5=CC=CC=C5C4(C)C)C)(C)C.[Cl-]
InChI InChI=1S/C47H66N5O3P.ClH/c1-35(2)52(36(3)4)56(54-34-20-32-48)55-38-30-28-37(29-31-38)49-45(53)27-14-11-19-33-51-42-24-18-16-22-40(42)47(7,8)44(51)26-13-10-12-25-43-46(5,6)39-21-15-17-23-41(39)50(43)9;/h10,12-13,15-18,21-26,35-38H,11,14,19-20,27-31,33-34H2,1-9H3;1H
InChIKey JTFPONRIAYGAKY-UHFFFAOYSA-N
Appearance Dark colored solid
CF260 0.03
CF280 0.04
Fluorescence Quantum Yield 0.2
Excitation 646
Emission 662
Storage Storage: 12 months after receival at -20 °C in the Dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Hydrogen Bond Donor Count 1
Hydrogen Bond Acceptor Count 7
Rotatable Bond Count 18
Exact Mass 815.4670057 g/mol
Monoisotopic Mass 815.4670057 g/mol
Topological Polar Surface Area 80.8Ų
Heavy Atom Count 57
Formal Charge 0
Complexity 1460
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 1
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 3
Covalently-Bonded Unit Count 2
Compound Is Canonicalized Yes

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