Aminoallyl-dUTP-XX-Texas Red

Aminoallyl-dUTP-XX-Texas Red is a Texas Red-labeled, aminoallyl-modified deoxyuridine triphosphate analog designed for fluorescent nucleic acid labeling. In common probe and assay workflows, the aminoallyl handle enables post-incorporation conjugation to amine-reactive dyes, affinity groups, or imaging handles, while the Texas Red fluorophore provides red fluorescence for microscopy and fluorescence readouts. This reagent is frequently used to generate fluorescently tagged DNA or RNA probes for nucleic acid analysis and molecular imaging experiments.

1. Fluorescent Nucleic Acid Probes

Aminoallyl-dUTP-XX-Texas Red is used to construct fluorescent DNA or RNA probes where Texas Red fluorescence provides direct visualization of hybridization or target-associated nucleic acid material. Researchers incorporate the analog during nucleic acid synthesis or nick translation workflows to introduce Texas Red signal into probe strands, then apply the resulting labeled probes in hybridization-based assays. The aminoallyl functionality supports downstream coupling steps when additional chemical handles are required for multicolor workflows, immobilization on surfaces, or attachment to carrier molecules used in probe handling and detection.

2. Fluorescence In Situ Hybridization

Aminoallyl-dUTP-XX-Texas Red supports fluorescence in situ hybridization (FISH) workflows that require robust red-channel labeling of nucleic acids in fixed biological samples or prepared nucleic acid preparations. The Texas Red fluorophore enables localization of target sequences within microscopy experiments, while the aminoallyl modification provides flexibility for additional conjugation steps when probe formats must be tuned for specific mounting, amplification, or signal management strategies. This reagent is therefore used by cell biology and molecular genetics laboratories to generate fluorescent probes for spatial mapping of DNA or RNA targets in microscopy imaging pipelines.

3. DNA Labeling For Hybridization Assays

Aminoallyl-dUTP-XX-Texas Red is applied in fluorescence-based nucleic acid hybridization assays where the fluorescent label is introduced into probe DNA to enable detection by plate readers, gel imaging systems, or fluorescence scanners. Laboratories use the Texas Red signal to monitor probe incorporation and to read out hybridization events in workflows such as probe-based binding studies and assay development for nucleic acid analysis. The aminoallyl handle is particularly useful when probe conjugates must be further functionalized after incorporation, for example to attach capture moieties for assay formats that rely on immobilized probe chemistry or to tailor probe behavior for downstream handling.

4. Post-Label Conjugate Construction

Aminoallyl-dUTP-XX-Texas Red is also valuable in workflows that require a two-step labeling strategy: first incorporating the aminoallyl-modified nucleotide into nucleic acid, then performing post-label coupling to install additional reagents for detection or immobilization. In practice, this enables construction of nucleic acid conjugates with Texas Red fluorescence plus an added chemical functionality, supporting custom probe architectures for research-grade molecular imaging and assay development. Teams developing specialized probe sets for multiplexing, surface immobilization, or conjugate-based detection often rely on the aminoallyl handle to expand compatibility with amine-reactive coupling partners used in fluorescence assay pipelines.