Aminoallyl-dUTP-XX-Cy5 (ethyl)

Name: Aminoallyl-dUTP-XX-Cy5 (ethyl)
Brand: BOC Sciences
Rating: 5 (1 reviews)

Catalog Number	A07-0034
Category	RNA/DNA Labeling
Molecular Formula	C57H80N7O23P3S2(freeacid)
Molecular Weight	1388.33

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Product Introduction

Aminoallyl-dUTP-XX-Cy5 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.

Chemical Information
Product Specification
Application

Chemical Information

Purity	≥95% (HPLC)
pH	7.5 ±0.5
Appearance	sterile blue solution in 10 mM Tris-HCl

Product Specification

Spectroscopic Properties	λexc 649 nm, λem 670 nm, ε 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Storage	store at-20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible.

Application

Aminoallyl-dUTP-XX-Cy5 (ethyl) is a Cy5-labeled nucleoside triphosphate designed for fluorescent incorporation into nucleic acids via enzymatic polymerization. The aminoallyl handle enables downstream conjugation for modular probe construction, while the Cy5 fluorophore provides red-emitting fluorescence for imaging and analytical readouts. This reagent is commonly used in nucleic acid labeling workflows where a defined dye-to-oligonucleotide architecture is required for fluorescence microscopy, hybridization-based assays, and fluorescence detection.

1. Oligonucleotide Fluorescent Labeling

Aminoallyl-dUTP-XX-Cy5 (ethyl) is used by molecular biology and chemical biology labs to generate fluorescently labeled DNA or RNA probes by enzymatic incorporation during nick translation, random priming, or polymerase-driven synthesis. Researchers rely on the Cy5 emission for red-channel fluorescence detection in hybridization experiments, probe-based binding studies, and fluorescence imaging of nucleic acid localization. The aminoallyl functionality supports flexible post-labeling strategies, allowing the labeled oligonucleotide to be further functionalized for specialized assay formats or conjugate assembly.

2. Fluorescent Hybridization Probes

Aminoallyl-dUTP-XX-Cy5 (ethyl) supports fluorescence-based nucleic acid detection where hybridization readouts are monitored by plate readers, gel documentation systems, or fluorescence imaging platforms. In probe development workflows, the Cy5 tag provides a robust signal for tracking target binding, while the aminoallyl group helps tailor the labeled probe for multiplexing strategies, surface immobilization, or conjugation to affinity reagents. This makes the reagent useful for developing hybridization probes used in research-grade nucleic acid analysis and molecular imaging reagent development.

3. Nucleic Acid Conjugate Construction

Aminoallyl-dUTP-XX-Cy5 (ethyl) is frequently selected for building fluorescent nucleic acid conjugates that require an additional reactive handle beyond the dye. Teams in biomaterials and chemical biology use the aminoallyl group to connect labeled oligonucleotides to carriers such as polymers, nanoparticles, or functionalized surfaces, enabling controlled presentation of fluorescent probes in sensing and imaging workflows. The result is a modular Cy5-containing nucleic acid reagent that can be engineered for specific assay architectures while maintaining a consistent fluorescent reporter for readout.