Aminoallyl-dUTP-XX-AF647

Aminoallyl-dUTP-XX-AF647 is a modified nucleoside building block designed for fluorescent labeling workflows, combining an aminoallyl-functionalized dUTP analog with an AF647 fluorophore handle. In nucleic acid applications, this reagent is used to introduce AF647 into DNA during enzymatic incorporation, enabling fluorescence-based readouts for microscopy, imaging, and nucleic acid analysis. The aminoallyl functionality supports downstream conjugation strategies when additional coupling chemistry is part of the labeling plan, while the AF647 dye provides strong far-red fluorescence for reduced spectral overlap in multicolor experiments.

1. Fluorescent DNA Labeling

Aminoallyl-dUTP-XX-AF647 is widely used to generate AF647-labeled DNA probes and fluorescent nucleic acid standards for hybridization and imaging workflows. Researchers incorporate the modified dUTP analog into DNA during enzymatic synthesis or labeling steps, producing AF647-tagged strands that can be visualized directly in fluorescence microscopy or used as probe reagents in nucleic acid hybridization assays. This format is particularly valuable when far-red emission is preferred to minimize interference from shorter-wavelength fluorophores and to support multichannel imaging designs.

2. Fluorescence In Situ Hybridization

Aminoallyl-dUTP-XX-AF647 supports fluorescence in situ hybridization workflows where AF647-labeled DNA probes are used to localize specific nucleic acid sequences in fixed samples. Molecular biology groups and cytogenetics laboratories commonly use AF647-labeled probes to visualize hybridization patterns with instrumentation such as fluorescence microscopes and automated imaging systems. The far-red dye is advantageous for imaging in complex biological backgrounds, where spectral separation from common cellular stains helps maintain clear signal assignment.

3. qPCR and Nucleic Acid Assay Probes

Aminoallyl-dUTP-XX-AF647 is also used in fluorescence-based nucleic acid assay development where AF647-labeled oligonucleotides are required as reporter components. In research settings, teams incorporate AF647-labeled nucleotides or generate AF647-containing probe oligos for assay formats that rely on fluorescence readout during nucleic acid workflows. This reagent is useful for building fluorescent probe reagents that integrate into established fluorescence detection platforms, including plate-based readers and fluorescence imaging systems used for nucleic acid analysis.

4. Sequencing and Library QC

Aminoallyl-dUTP-XX-AF647 can be leveraged for fluorescence-based quality control and characterization of nucleic acid libraries and labeled DNA constructs. Molecular profiling and genomics research groups use AF647-tagged nucleic acid material to verify labeling consistency, monitor incorporation during library preparation, or support downstream visualization steps in workflows that require a robust far-red fluorescence reporter. The reagent's nucleoside compatibility with enzymatic nucleic acid handling makes it a practical tool for generating fluorescently traceable DNA materials for process monitoring and analytical confirmation.