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Aminoallyl-dUTP-XX-AF488
| Catalog Number | A07-0020 |
| Category | RNA/DNA Labeling |
| Molecular Formula | C45H54N7O26P3S2(freeacid) |
| Molecular Weight | 1265.99 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A07-0020 | -- | $-- |
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Product Introduction
Aminoallyl-dUTP-XX-AF488 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. AF488 (also known as Alexa Fluor 488®) is a hydrophilic dye with excellent photostability compared to fluorescein.
Chemical Information |
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|---|---|
| Purity | ≥95% (HPLC) |
| pH | 7.5 ±0.5 |
| Appearance | sterile yellow solution in 10 mM Tris-HCl |
- Product Specification
| Spectroscopic Properties | λexc 494 nm, λem 515 nm, ε 73.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) |
| Storage | store at-20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible. |
Applications of Fluorescent Probes & Dyes
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