Aminoallyl-dUTP-ATTO-655

Aminoallyl-dUTP-ATTO-655 is an ATTO 655-labeled nucleotide analog designed for post-incorporation fluorescent tagging of nucleic acids. The aminoallyl functionality enables straightforward conjugation to activated dyes or reactive handles, while the ATTO 655 fluorophore provides a far-red emission signal commonly leveraged for fluorescence-based nucleic acid analysis and imaging workflows.

1. DNA/RNA Labeling

Aminoallyl-dUTP-ATTO-655 is used by molecular biology and genomics teams to generate fluorescently labeled DNA or RNA probes by incorporating the analog during nucleic acid synthesis. Researchers employ the ATTO 655 signal for downstream fluorescence readouts such as hybridization-based detection, microscopy of labeled nucleic acids, and traceable nucleic acid tracking in assay development. The aminoallyl handle supports flexible downstream functionalization strategies when additional conjugation steps are required for probe construction or multi-label experiments.

2. Fluorescent Hybridization Probes

Aminoallyl-dUTP-ATTO-655 is frequently incorporated into oligonucleotide or nucleic acid probe preparations used in fluorescence hybridization workflows. In research settings, labeled probes are used to visualize or quantify nucleic acid targets after hybridization, supporting applications such as blot-based detection, probe-based imaging, and assay development where far-red fluorescence helps reduce spectral overlap with common green channels. The ATTO 655 emission is particularly useful when multiplexing requires careful separation between fluorophores.

3. Nucleic Acid Microarray Imaging

Aminoallyl-dUTP-ATTO-655 is applied in microarray and array-based nucleic acid workflows where fluorescent labeling is required for scanning readout. By integrating the ATTO 655-bearing nucleotide analog into probe or target preparations, laboratories can generate high-contrast far-red signals compatible with standard fluorescence microarray scanners. This approach is commonly used in screening and validation experiments that rely on robust, image-based quantification of hybridization patterns.

4. Fluorescence Microscopy Tracking

Aminoallyl-dUTP-ATTO-655 supports fluorescence microscopy studies that visualize nucleic acids in fixed or prepared samples, enabling spatial mapping of labeled DNA/RNA species. Researchers use the far-red ATTO 655 channel to track nucleic acid localization patterns while maintaining compatibility with multi-color imaging panels that often include green and red fluorophores. The aminoallyl-enabled labeling strategy also supports workflows where additional chemical handles are desired for custom probe architectures.