Aminoallyl-dUTP-ATTO-550

Aminoallyl-dUTP-ATTO-550 is an aminoallyl-functionalized nucleoside analog bearing ATTO 550, a bright red/orange-emitting fluorophore commonly used for fluorescence labeling of nucleic acids. The aminoallyl handle enables post-incorporation conjugation to amine-reactive dyes, affinity tags, or other biomolecular linkers, making it a practical reagent for constructing fluorescent DNA/RNA probes and labeled templates for imaging and analysis workflows. Its incorporation as a dUTP analog supports enzymatic incorporation into nucleic acids, while the ATTO 550 fluorophore provides direct fluorescence readout for microscopy, spectroscopy, and flow-based detection platforms.

1. Fluorescent Nucleic Acid Labeling

Aminoallyl-dUTP-ATTO-550 is widely used in fluorescence labeling of DNA and RNA for downstream imaging and analytical assays where an ATTO 550 signal is tracked. Researchers incorporate the dUTP analog during enzymatic synthesis or labeling workflows to generate fluorescently tagged nucleic acid samples suitable for gel-based visualization, fluorescence scanning, and probe-based hybridization experiments. The aminoallyl functionality offers an additional conjugation opportunity for building customized fluorescent nucleic acid reagents, including adding secondary labels for multicolor workflows or attaching capture moieties for assay development.

2. Nucleic Acid Probe Construction

Aminoallyl-dUTP-ATTO-550 supports the development of fluorescent oligonucleotide probes used to monitor hybridization events and to map nucleic acid targets in molecular biology research. Teams constructing labeled probe sets for microscopy-based localization studies or fluorescence readouts in nucleic acid assays benefit from the stable, high-contrast ATTO 550 emission and the aminoallyl handle for optional post-labeling modifications. This combination is particularly useful when probes need to be further functionalized for immobilization on surfaces, incorporation into imaging workflows, or integration into assay formats that require a defined fluorescent tag density.

3. Fluorescence In Situ Hybridization Workflows

Aminoallyl-dUTP-ATTO-550 is frequently employed in fluorescence in situ hybridization (FISH) and related hybridization-based microscopy workflows where fluorescent nucleic acid signals are localized to cells, chromosomes, or specific nucleic acid regions. Laboratory groups value the ATTO 550 fluorophore for producing robust red/orange fluorescence that can be combined with other spectral channels in multicolor staining panels. The aminoallyl group also provides a route to tailor probe conjugation strategies when building multi-component labeling schemes or when additional chemical handles are required for consistent probe preparation.

4. Flow Cytometry Nucleic Acid Analysis

Aminoallyl-dUTP-ATTO-550 is used in fluorescence-based nucleic acid analysis workflows where labeled nucleic acids are quantified by flow cytometry or related single-particle fluorescence measurements. Researchers prepare fluorescently tagged nucleic acid reagents to track hybridization, uptake, or nucleic acid-associated signals in cell-associated or bead-associated formats, leveraging the ATTO 550 emission for gating and quantification. The aminoallyl functionality can be leveraged during reagent design to enable conjugation to carriers or to standardize labeling strategies across probe batches used in high-throughput screening and assay development.