Aminoallyl-dUTP-ATTO-550

Product Introduction

Aminoallyl-dUTP-ATTO550 is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.
Recommended Aminoallyl-dUTP-ATTO550/dTTP ratio Nick Translation: 30-50% Aminoallyl-dUTP-ATTO550/ 50% dTTP

Chemical Information
Purity	≥95% (HPLC)
pH	7.5 ±0.5
Appearance	sterile red-violet solution in 10 mM Tris-HCl

Product Specification
Spectroscopic Properties	λexc 554 nm, λem 576 nm, ε 120.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Storage	store at-20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible.