Inquiry Basket
Meta-iodoHoechst 33258 | 158013-42-4
| Catalog Number | A19-0052 |
| Category | DNA Stains |
| Molecular Formula | C25H23IN6 |
| Molecular Weight | 534.39 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A19-0052 | -- | $-- |
- Worldwide Delivery
- Quality Assurance
- 24/7 Customer Service
Product Introduction
Meta-iodoHoechst 33258, an iodinated DNA-binding bibenzimidazole, is a cell dye for DNA quantitation which sensitizes DNA and cells to UVA.
Chemical Information |
|
|---|---|
| Synonyms | 2'-(3-Iodophenyl)-5-(4-methyl-1-piperazinyl)-2,5'-BI-benzimidazole |
| Shelf Life | 2 years |
| IUPAC Name | 2-(3-iodophenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole |
| Canonical SMILES | CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)N=C(N5)C6=CC(=CC=C6)I |
| InChI | InChI=1S/C25H23IN6/c1-31-9-11-32(12-10-31)19-6-8-21-23(15-19)30-25(28-21)17-5-7-20-22(14-17)29-24(27-20)16-3-2-4-18(26)13-16/h2-8,13-15H,9-12H2,1H3,(H,27,29)(H,28,30) |
| InChI Key | ZEXOKHKEQNNOBG-UHFFFAOYSA-N |
| Density | 1.587±0.06 g/cm3 |
| Appearance | Solid Powder |
- Product Specification
- Application
| Storage | Store at -20°C |
Meta-iodoHoechst 33258, a versatile dye commonly utilized in bioscience, offers a myriad of applications owing to its capability to stain DNA and its fluorescent attributes. Here are four key applications of Meta-iodoHoechst 33258:
Cellular Imaging: Embraced in fluorescence microscopy, Meta-iodoHoechst 33258 emerges as a prominent tool for staining cell nuclei. With a unique affinity for the minor groove of DNA, this dye enables researchers to visualize and quantify cell nuclei with precision and sharp contrast. Its pivotal role extends to cell cycle investigations, apoptosis studies, and cancer diagnostics, unveiling the intricacies of cellular behaviors with striking clarity.
Flow Cytometry: Within the realm of flow cytometry, Meta-iodoHoechst 33258 shines as a DNA-staining powerhouse, empowering the analysis of cell populations based on DNA content. This staining agent plays a critical role in deciphering cell cycle distribution, identifying aneuploid cells, and evaluating cellular DNA damage. Facilitating seamless high-throughput analysis and cell sorting, it serves as a valuable asset across diverse research and clinical endeavors.
Chromatin Structure Studies: Delving into the exploration of chromatin structure and organization, Meta-iodoHoechst 33258 reveals its prowess. By intricately staining chromatin and subjecting it to visualization under a fluorescence microscope, researchers can delve into the dynamics, compaction, and interactions of chromatin with other nuclear components. This insightful process sheds light on gene regulation, epigenetics, and the impacts of various treatments on chromatin architecture, enriching our understanding of cellular mechanisms.
DNA Quantification: Beyond its imaging capabilities, Meta-iodoHoechst 33258 plays a vital role in spectrophotometric assays for DNA quantification. When bound to DNA, it amplifies fluorescence levels, enabling precise measurement of DNA concentration in samples. This functionality finds utility across a spectrum of applications, from advancing molecular biology experiments to supporting clinical diagnostics and forensic analyses, underscoring its indispensability in diverse scientific pursuits.
Computed Properties | |
|---|---|
| XLogP3 | 5 |
| Hydrogen Bond Donor Count | 2 |
| Hydrogen Bond Acceptor Count | 4 |
| Rotatable Bond Count | 3 |
| Exact Mass | 534.10289 g/mol |
| Monoisotopic Mass | 534.10289 g/mol |
| Topological Polar Surface Area | 63.8Ų |
| Heavy Atom Count | 32 |
| Formal Charge | 0 |
| Complexity | 642 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
Applications of Fluorescent Probes & Dyes
Get More Product Details?
