Hoechst 33258 Analog 6 | CAS 129244-66-2

Catalog Number	A19-0047
Category	DNA Stains
Molecular Formula	C33H40N6O
Molecular Weight	536.71

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Product Introduction

Hoechst 33258 analog 6 is an analog of Hoechst 33258 that can be used for staining DNA as a blue fluorescent dye.

Chemical Information
Product Specification
Application

Synonyms	2,6-di-tert-butyl-4-[6-[6-(4-methylpiperazin-1-yl)-1-H-benzimidazol-2-yl]-1-H-benzimidazol-2-yl]phenol
Purity	≥98%
Shelf Life	2 years
Canonical SMILES	CC(C)(C)C1=CC(=CC(=C1O)C(C)(C)C)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C
InChI	InChI=1S/C33H40N6O/c1-32(2,3)23-16-21(17-24(29(23)40)33(4,5)6)31-35-25-10-8-20(18-27(25)36-31)30-34-26-11-9-22(19-28(26)37-30)39-14-12-38(7)13-15-39/h8-11,16-19,40H,12-15H2,1-7H3,(H,34,37)(H,35,36)
InChIKey	LXRWWFIBSFWADZ-UHFFFAOYSA-N
Appearance	Solid Powder

Storage

Store at -20°C

Hoechst 33258 Analog 6, a fluorescent dye widely utilized in diverse biological and clinical contexts, offers an array of applications showcased below:

Cell Nuclei Staining: Widely employed in fluorescence microscopy, Hoechst 33258 Analog 6 is a staple for staining cell nuclei. Emitting a vibrant blue fluorescence upon DNA binding, it enables researchers to vividly visualize and quantify nuclear morphology and DNA content. This pivotal application plays a vital role in cell cycle investigations, apoptosis assays, and chromosome analyses, shedding light on intricate cellular processes.

Live Cell Imaging: Renowned for its low toxicity and strong DNA affinity, Hoechst 33258 Analog 6 is a go-to option for live cell imaging. Research endeavors benefit from the ability to observe real-time cellular dynamics, including cell division, migration, and nuclear activities, without compromising cell viability significantly. This capability is especially crucial for dynamic studies in developmental biology and cancer research, offering a glimpse into the dynamic nature of living cells.

Flow Cytometry: Embraced in flow cytometry, Hoechst 33258 Analog 6 plays a pivotal role in analyzing cellular DNA content. Its usage allows for the discrimination of distinct cell populations based on their DNA content, facilitating cell cycle assessments and the identification of stem cells or specific subpopulations. This versatile application garners significance in both fundamental research and clinical diagnostics, aiding in unraveling the intricacies of cellular composition and function.

Genotoxicity Testing: Leveraged in genotoxicity assays, Hoechst 33258 Analog 6 serves as a valuable tool in detecting DNA damage induced by potential mutagens. By staining DNA, researchers can evaluate chromosomal aberrations, micronuclei formation, and other genotoxic effects under fluorescence microscopy. This critical function plays a pivotal role in assessing the safety and hazards of new chemicals and pharmaceuticals.