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Hoechst 33258 analog 5 | 23491-55-6
| Catalog Number | A19-0057 |
| Category | DNA Stains |
| Molecular Formula | C29H26N6 |
| Molecular Weight | 458.56 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A19-0057 | -- | $-- |
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Product Introduction
Hoechst 33258 analog 5 is a blue fluorescent dye used for staining DNA.
Chemical Information |
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|---|---|
| Synonyms | 6-(4-methylpiperazin-1-yl)-2-(2-naphthalen-2-yl-3-H-benzimidazol-5-yl)-1-H-benzimidazole |
| Shelf Life | 2 years |
| Canonical SMILES | CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)N=C(N5)C6=CC7=CC=CC=C7C=C6 |
| InChI | InChI=1S/C29H26N6/c1-34-12-14-35(15-13-34)23-9-11-25-27(18-23)33-29(31-25)22-8-10-24-26(17-22)32-28(30-24)21-7-6-19-4-2-3-5-20(19)16-21/h2-11,16-18H,12-15H2,1H3,(H,30,32)(H,31,33) |
| InChI Key | FJXFKDPHPUCTJZ-UHFFFAOYSA-N |
| Density | 1.314±0.06 g/cm3 |
| Appearance | Solid Powder |
| Boiling Point | 766.3±70.0 °C | Condition: Press: 760 Torr |
- Product Specification
- Application
| Storage | Store at -20°C |
Hoechst 33258 analog 5, a fluorescent dye renowned for its DNA staining capabilities in diverse bioscience applications, plays a pivotal role in various areas. Here are four key applications:
Cell Cycle Analysis: Employing Hoechst 33258 analog 5 in fluorescence-activated cell sorting (FACS) facilitates the meticulous analysis of cell cycle phases. By imbuing DNA with its distinct fluorescence, researchers can quantitatively assess the DNA content of individual cells, pinpointing the specific phase of the cell cycle each cell occupies. This process is crucial for delving into cell proliferation dynamics and comprehending the intricate workings of the cell cycle.
Chromatin Structure Studies: Delving into the realm of chromatin organization and structure, this illuminative dye binds to the minor groove of DNA, allowing for the visualization of chromatin within cells under a fluorescence microscope. This indispensable application sheds light on epigenetic modifications and nuclear architecture, offering insights into the intricate world of chromatin structure exploration.
High-Throughput Screening: Leveraging the prowess of Hoechst 33258 analog 5 in high-throughput screening assays empowers researchers to swiftly pinpoint compounds that exert an influence on DNA. By staining DNA in microplate formats, scientists can rapidly evaluate the impact of diverse drugs or genetic perturbations on cellular DNA, expediting the discovery of novel therapeutic agents and enhancing genotoxicity testing protocols.
Apoptosis Detection: An integral tool for apoptosis detection, Hoechst 33258 analog 5 aids in identifying changes in nuclear morphology indicative of programmed cell death. During apoptosis, cells exhibit characteristic chromatin condensation and fragmentation, which are readily discernible when stained with this dye. This application fuels research into programmed cell death mechanisms and its implications in conditions like cancer, offering valuable insights into the cellular processes underlying disease progression.
Applications of Fluorescent Probes & Dyes
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