Hoechst 33258 analog 3 | 23554-98-5

Hoechst 33258 analog 3, a renowned fluorescent dye in bioscience research for DNA staining, finds diverse applications. Here are four key uses:

Cell Cycle Analysis: A staple in cell cycle studies, Hoechst 33258 analog 3 intricately binds to DNA’s A-T rich regions, aiding researchers in visualizing distinct cell cycle phases via fluorescence microscopy or flow cytometry. This enables precise quantification of cell proliferation and deep insights into cell cycle dynamics, enhancing our understanding of cellular behavior.

Apoptosis Detection: In the realm of apoptosis, Hoechst 33258 analog 3 shines as a beacon for detecting DNA fragmentation, a hallmark of programmed cell death. By binding to fragmented DNA and emitting a distinguishable fluorescence signal, this dye unveils apoptotic cells, offering researchers a window into the mechanisms of cell demise and facilitating the screening of apoptosis-inducing compounds in drug discovery.

Chromatin and Nuclear Structure Studies: Delving into nuclear and chromatin realms, Hoechst 33258 analog 3 proves indispensable for staining and visualizing these intricate structures. Its strong DNA affinity makes it a vital probe for exploring nuclear architecture, chromatin condensation, and shifts in nuclear morphology. Through these studies, researchers gain valuable insights into genome organization and the role of nuclear structure in diverse biological processes.

Fluorescence Microscopy: A cornerstone in fluorescence microscopy, Hoechst 33258 analog 3 elevates the imaging of nucleic acids in live or fixed cells. Emitting a vibrant blue fluorescence upon DNA binding, this dye enables clear visualization of cell nuclei, essential for a range of cellular and molecular biology investigations, from cytogenetics to tissue imaging. This application aids in uncovering intricate details at the subcellular level, driving forward scientific discovery.