Hoechst 33258 analog 2 | 23491-54-5

Hoechst 33258 analog 2, a widely utilized fluorescent dye, finds its application in diverse biological contexts. Here are four key applications:

Cell Cycle Analysis: Harnessing Hoechst 33258 analog 2 in flow cytometry unlocks the ability to delve into the intricate phases of the cell cycle. By staining nuclear DNA, researchers unveil the DNA content of individual cells, distinguishing between cells residing in G0/G1, S, and G2/M phases. This technique serves as a cornerstone for scrutinizing cell proliferation, apoptosis, and the impacts of various treatments on cell cycle progression.

Microscopy: Enabling precise visualization of DNA within cells, Hoechst 33258 analog 2 is a cornerstone of fluorescence microscopy. Its blue fluorescence delicately stains nuclei, offering clear insights into nuclear morphology and chromosomal structures. Particularly valuable in immunofluorescence studies, where nuclear counterstaining complements other fluorescent markers, this application elevates the quality of cellular imaging studies.

Chromosome Analysis: At the forefront of cytogenetics, Hoechst 33258 analog 2 plays a pivotal role in chromosome banding techniques like Q-banding. By selectively binding to AT-rich regions of DNA, it generates distinct banding patterns on metaphase chromosomes, aiding in the identification of chromosomal abnormalities. This critical application is indispensable in clinical diagnostics and genetic research for unraveling chromosomal rearrangements and aneuploidies.

Drug Screening: Adapting Hoechst 33258 analog 2 for high-throughput screening assays revolutionizes the evaluation of chemical compounds’ genotoxic potential. By scrutinizing alterations in nuclear morphology and DNA integrity, researchers pinpoint compounds triggering DNA damage or other genotoxic effects. This proactive approach not only assists in the early-phase development of pharmaceuticals but also bolsters safety assessments of new chemical entities.