HOE-S 785026 | 132869-83-1

HOE-S 785026 is a highly effective cell-permeable fluorescent dye that binds to DNA, allowing researchers to visualize and quantify nucleic acids in various biological samples. Known for its high affinity and specificity for DNA, HOE-S 785026 has become a critical tool in molecular biology, particularly in fields that require precise DNA visualization. As a fluorescent dye, its ability to penetrate cell membranes without causing significant disruption makes it a versatile candidate for numerous applications, providing clear, bright signals that facilitate accurate data analysis.

One of the primary applications of HOE-S 785026 is in flow cytometry, where it is used to stain DNA and assess cell cycle distributions. This application is vital for understanding cellular processes, including proliferation, apoptosis, and DNA repair mechanisms. By staining cells with HOE-S 785026, researchers can accurately determine the stages of the cell cycle, which is essential for studies in cancer research and drug discovery, offering valuable insights into how cells respond to various treatments at different cycle stages.

Another key application of HOE-S 785026 is in fluorescence microscopy, where it enables the visualization of nuclear DNA in fixed or live cells. This application is crucial for studying cellular and nuclear morphology and for identifying structural changes over time. The dye’s strong fluorescence helps in producing high-resolution images that can reveal important details about nuclear organization, chromatin structure, and abnormal morphological changes associated with diseases such as cancer.

HOE-S 785026 is also employed in gel electrophoresis for the purpose of detecting and quantifying DNA fragments. After electrophoresis, the dye can bind to DNA within agarose or polyacrylamide gels, allowing visualization under UV light. This method is commonly used in genetic engineering, cloning, and molecular diagnostics, where precise DNA analysis is required. The fluorescence provided by HOE-S 785026 ensures that even small quantities of DNA can be detected and assessed efficiently.

Lastly, HOE-S 785026 finds application in apoptosis studies, where it is used to distinguish between live, apoptotic, and necrotic cells. By differentially staining cells based on membrane integrity and DNA content, researchers can effectively study programmed cell death mechanisms. It’s especially helpful in understanding disease pathogenesis and the effects of therapeutic interventions, making it an invaluable tool in both basic research and clinical settings.