FAM-11-dCTP, 6-isomer

FAM-11-dCTP, 6-isomer is a fluorescein (FAM)-labeled deoxycytidine triphosphate designed for incorporation into nucleic acids during polymerase-driven synthesis. The fluorescein reporter enables fluorescence readout for labeled DNA/RNA generation, while the triphosphate functionality supports enzymatic uptake into templates in common nucleic acid workflows. This reagent is frequently used when fluorescent tagging of cytosine positions is needed for visualization, quantification, or downstream probe construction.

1. Fluorescent DNA Labeling

FAM-11-dCTP, 6-isomer is used by molecular biology and nucleic acid analysis teams to introduce fluorescein into DNA during PCR, nick translation, or other polymerase-mediated extension reactions where cytosine analog incorporation is acceptable. Labeled products are then analyzed by fluorescence imaging or fluorescence-based quantification, including gel documentation and fluorescence scanners, enabling researchers to track amplification or to generate fluorescent DNA standards for assay development. The fluorescein tag provides a convenient optical handle for workflows that require covalent, positionally distributed labeling rather than post-labeling dyes.

2. Fluorescent Probe Construction

FAM-11-dCTP, 6-isomer supports the generation of fluorescent DNA probes by enzymatic synthesis of labeled oligonucleotides or probe fragments that can be used for hybridization-based detection formats. In research settings, teams use fluorescein incorporation to create probes for nucleic acid hybridization experiments where the probe signal is read directly by fluorescence instrumentation. This approach is particularly useful for building probe libraries or optimizing hybridization workflows, since the fluorescent label is built into the probe during synthesis rather than relying on separate dye conjugation steps.

3. Fluorescence-Based Nucleic Acid Assays

FAM-11-dCTP, 6-isomer is applied in fluorescence readout assays that rely on incorporation of fluorescent nucleotides to monitor nucleic acid synthesis or processing. Assay developers use the reagent to generate fluorescently labeled DNA/RNA substrates for method development, signal mapping, and comparative evaluation of polymerase performance in fluorescence-based measurement contexts. The triphosphate form aligns with nucleic acid enzymology workflows, allowing researchers to tune labeling density through incorporation strategy while maintaining a nucleic-acid-compatible reagent format for fluorescence detection.