DyeTOX 13 Green Fluorescent Nucleic Acid Stain

DyeTOX 13 Green Fluorescent Nucleic Acid Stain is a green-emitting fluorescent nucleic acid stain designed for visualization of DNA and RNA in common fluorescence workflows. Its nucleic-acid affinity enables straightforward staining of nucleic-acid-containing samples for microscopy- and plate-based readouts, supporting routine nucleic acid detection and nucleic-acid integrity or presence assessments in research and assay development environments.

1. Fluorescence Microscopy Staining

DyeTOX 13 Green Fluorescent Nucleic Acid Stain is used to visualize nucleic acids in fixed-cell or prepared sample formats where DNA/RNA localization or nucleic-acid presence is of interest. Researchers employ the stain in fluorescence microscopy to generate green-channel contrast for nucleic-acid-containing regions, supporting workflows such as staining of nucleic-acid-rich specimens, qualitative assessment of sample preparation, and imaging of nucleic-acid-containing biomaterials. The green emission profile aligns well with standard visible-light fluorescence microscopes equipped with appropriate filter sets for green fluorophores, enabling routine imaging without specialized instrumentation.

2. Gel Documentation And Electrophoresis Imaging

DyeTOX 13 Green Fluorescent Nucleic Acid Stain is frequently adopted for fluorescence-based nucleic acid visualization in electrophoresis workflows, including agarose and polyacrylamide gel imaging. In molecular biology and analytical chemistry laboratories, the stain is used to monitor DNA or RNA bands after separation, supporting tasks such as checking fragment distribution, confirming successful amplification or restriction workflows, and documenting nucleic-acid migration patterns using gel documentation systems with compatible excitation/emission optics. This application is particularly valued for its sensitivity to nucleic acids and its compatibility with routine lab imaging pipelines.

3. Nucleic Acid Quantification Assays

DyeTOX 13 Green Fluorescent Nucleic Acid Stain is applied in fluorescence-based nucleic acid quantification and presence/absence screening in assay development and routine lab testing. Researchers use the stain in plate-based formats to read out nucleic-acid-associated fluorescence, enabling throughput-friendly monitoring of nucleic acid samples such as purified DNA/RNA, normalized library inputs, or nucleic-acid-containing reaction mixtures. The green fluorescent signal supports integration into standard microplate readers configured for visible fluorescence measurements, making the stain useful for workflow standardization during nucleic acid handling and method development.

4. Flow Cytometry Nucleic Acid Readouts

DyeTOX 13 Green Fluorescent Nucleic Acid Stain is used in flow cytometry workflows where nucleic-acid-associated fluorescence provides a readout for nucleic-acid content in cell populations or cell-associated nucleic-acid preparations. In chemical biology and biomaterials research settings, investigators apply the stain to generate green-channel signal that can be used to gate populations based on nucleic-acid staining intensity, supporting studies that involve nucleic-acid-containing materials, transfection-related sample comparisons, or nucleic-acid signal tracking in prepared suspensions. Compatibility with common flow cytometry detection configurations for green fluorophores enables integration into established cytometer analysis pipelines.