BOBO-3 Iodide (570/602)

BOBO-3 Iodide (570/602) is a nucleic-acid-selective fluorescent dye designed for red/orange excitation and far-red emission, commonly used to visualize DNA and RNA in fluorescence-based workflows. Its strong staining behavior and compatibility with standard fluorescence instrumentation make it a practical reagent for nucleic acid staining, imaging, and assay development where robust signal from nucleic acids is required. The iodide salt form supports convenient handling in staining and labeling protocols.

1. DNA And RNA Staining

BOBO-3 Iodide (570/602) is widely used as a nucleic-acid stain for fluorescence microscopy and gel-based visualization of DNA and RNA. Researchers typically incorporate the dye into staining steps to generate bright contrast from nucleic acid-containing samples, including electrophoresis gels, nucleic-acid gels, and stained nucleic-acid preparations for downstream imaging. The dye's red-shifted emission is particularly helpful when separating nucleic-acid signal from background autofluorescence or when working in multi-color experiments that include shorter-wavelength fluorophores.

2. Fluorescence Microscopy Imaging

BOBO-3 Iodide (570/602) supports cellular and molecular imaging workflows that require nucleic-acid visualization in the same imaging session as other fluorescence readouts. In microscopy experiments, users apply the dye to label DNA/RNA in fixed or prepared samples to map nucleic-acid distribution patterns and to support qualitative localization studies. The far-red emission profile also aligns well with common microscope filter sets used for red-channel detection, enabling straightforward integration into established imaging pipelines.

3. Flow Cytometry Nucleic Acid Readouts

BOBO-3 Iodide (570/602) is used in flow cytometry workflows where fluorescence intensity correlates with nucleic-acid content, supporting cell-cycle and nucleic-acid content analysis in research settings. Laboratories employ the dye as a DNA/RNA staining reagent to generate cytometric distributions and to support gating strategies based on nucleic-acid-associated fluorescence. Its emission in the far-red region helps reduce spectral overlap with many green-emitting dyes, simplifying panel design for multi-parameter cytometry experiments.

4. Fluorescent Nucleic Acid Assays

BOBO-3 Iodide (570/602) is frequently incorporated into fluorescence-based nucleic-acid assay development, including protocols that rely on nucleic-acid staining as the primary readout. Assay developers use the dye to monitor nucleic-acid presence and relative abundance in plate-based formats, imaging assays, and analytical workflows where nucleic-acid fluorescence provides the measurement signal. The dye's compatibility with standard fluorescence detection channels makes it a useful component for screening nucleic-acid samples and optimizing staining conditions for laboratory assays.