BOBO-1 Iodide (462/481)

BOBO-1 Iodide (462/481) is a DNA-binding fluorescent dye designed for nucleic acid staining and fluorescence-based analysis, with excitation and emission well matched to common blue-light excitation and visible detection channels. Its strong affinity for nucleic acids makes it useful for visualizing DNA in gel and microscopy workflows, as well as for fluorescence readouts in nucleic acid assays where a high-contrast nucleic acid stain is required.

1. DNA Gel Staining

BOBO-1 Iodide (462/481) is frequently used as a fluorescent stain for agarose gel electrophoresis to visualize DNA bands with blue-light/visible fluorescence imaging systems. Researchers and core facilities use it to streamline gel documentation by replacing traditional DNA stains, enabling rapid band visualization and downstream imaging workflows that rely on standard fluorescence gel imagers.

2. Fluorescence Microscopy DNA Staining

BOBO-1 Iodide (462/481) supports fluorescence microscopy workflows where DNA localization or nucleic acid distribution is visualized in fixed samples or nucleic-acid-containing preparations. Cell biology and molecular imaging laboratories use it to stain DNA in microscopy experiments, including studies that require clear nuclear contrast and compatibility with fluorescence imaging setups tuned to the dye's excitation/emission window.

3. Nucleic Acid Quantification Assays

BOBO-1 Iodide (462/481) is applied in fluorescence-based nucleic acid quantification and nucleic-acid monitoring assays where dye-DNA binding produces a measurable fluorescence signal. Molecular biology labs and assay developers use it in plate-based or cuvette-based fluorescence measurements to track DNA presence and relative nucleic acid abundance during workflow development, buffer screening, or sample quality checks.

4. Flow Cytometry DNA Content Analysis

BOBO-1 Iodide (462/481) is used in flow cytometry protocols that require DNA staining for fluorescence readout of nucleic-acid content in stained cell populations. Cytometry users in academic and industrial research environments incorporate it into staining workflows for DNA-content distributions, leveraging its nucleic acid binding behavior to generate fluorescence signals compatible with instruments that detect emission within the dye's visible range.