Phalloidin-ATTO 740

Phalloidin-ATTO 740 is a fluorescent phalloidin conjugate designed for actin visualization, combining the actin-binding selectivity of phalloidin with an ATTO 740 fluorophore for far-red fluorescence detection. In microscopy and imaging workflows, this reagent is used to label filamentous actin (F-actin) in fixed cells and to support quantitative analysis of cytoskeletal organization with instrumentation capable of far-red excitation and emission collection.

1. F-Actin Fluorescence Microscopy

Phalloidin-ATTO 740 is widely used in fluorescence microscopy workflows to stain filamentous actin structures such as stress fibers, cortical actin, and organized cytoskeletal networks in fixed cell preparations. Researchers in cell biology and chemical biology use the far-red ATTO 740 signal to visualize actin architecture with reduced spectral overlap when combined with other fluorophores, supporting multi-channel imaging of cell morphology, adhesion phenotypes, and cytoskeletal remodeling after experimental perturbations.

2. Cytoskeleton Quantification Assays

Phalloidin-ATTO 740 enables quantitative image-based readouts of actin organization by providing a strong, spatially resolved fluorescence signal that can be analyzed by automated microscopy pipelines. Cell imaging groups and assay developers use this reagent to compare actin filament density, alignment, and subcellular distribution across experimental conditions, including studies of cytoskeletal dynamics and actin-targeting reagents, where consistent staining of F-actin is required for robust downstream image analysis.

3. Multiplex Imaging With Far-Red Channel

Phalloidin-ATTO 740 is commonly incorporated into multiplex fluorescence panels where a far-red excitation/emission window is advantageous for separating actin labeling from other cellular markers. Imaging specialists use the ATTO 740 channel to reduce cross-talk in multi-target staining experiments, including co-localization studies that pair actin visualization with membrane markers, organelle dyes, or nuclear stains, leveraging the stable far-red reporting to improve interpretability of multi-color datasets.

4. Fixed-Cell Actin Staining Panels

Phalloidin-ATTO 740 is used to build standardized fixed-cell staining protocols for cytoskeleton-focused workflows in research laboratories. Microscopy and screening teams apply the reagent to generate reproducible actin labeling across batches of fixed samples, supporting comparative studies in cell culture models where filamentous actin visualization is a primary endpoint and where far-red fluorescence collection is aligned with the available microscope filter sets or detector configurations.