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Product Introduction
ATTO 665 is a new fluorescent label closely related to ATTO 647N. It shows extraordinary high fluorescence quantum yield, excellent thermal and photo-stability, outstanding ozone resistance, and very little triplet formation. ATTO 665 is a cationic (charge +1). The dye is moderately hydrophilic. The NHS-ester of the dye reacts easily with amino-groups of proteins and other bio-molecules. Since the amino group must be non protonated to be reactive, the pH of the reaction is recommended to be about 8.3. Prepare labeling solution of NHS-ester immediately before use by dissolving the vial content in anhydrous and amine-free DMF or DMSO.find more information here
Chemical Information
Application
| NACRES | NA.32 |
ATTO 665 NHS ester, a high-performance fluorescent dye, finds diverse applications in biotechnological and medical arenas. Here, discover four key applications of ATTO 665 NHS ester:
Fluorescence Microscopy: Embracing ATTO 665 NHS ester in fluorescence microscopy empowers researchers to intricately label proteins, nucleic acids, and other biomolecules. Its luminous fluorescence and enduring photostability enable the visualization and meticulous tracking of intricate cellular processes with exceptional precision. This staining agent proves particularly invaluable for prolonged imaging experiments, where concerns of photobleaching loom large.
Flow Cytometry: Within flow cytometry assays, ATTO 665 NHS ester takes center stage for cell labeling, sorting, and analysis. Boasting intense fluorescence output and seamless compatibility with standard flow cytometry equipment, it emerges as the ideal tool for multiparametric cellular investigations. This application aids in the exhaustive exploration of cell populations, facilitating the pinpointing of specific cell types amid mixed cohorts.
FRET Imaging: Embarking on Förster Resonance Energy Transfer (FRET) explorations calls for the incorporation of ATTO 665 NHS ester as an eminent acceptor dye, courtesy of its distinct emission spectrum. When paired with a fitting donor dye, it unlocks the realm of nanometer-scale examinations into intermolecular interactions. This transformative application stands as a linchpin for delving into protein-protein couplings, conformational shifts, and assorted molecular dynamics.
Western Blotting: In the realm of Western blot assays, ATTO 665 NHS ester emerges as a formidable ally for antibody labeling. Its robust fluorescent signal amplifies the detection of target proteins on membranes with acute sensitivity and minimal background noise. This enhancement significantly bolsters the precision and dependability of protein quantification and identification across varied research undertakings.
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