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Anti-Rabbit IgG-ATTO 633 antibody produced in goat
| Catalog Number | F10-0144 |
| Category | ATTO Dyes |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Anti-rabbit IgG (whole molecule) (Sigma, R4880) is developed in goat using purified rabbit IgG as the immunogen. Affinity isolated antigen specific antibody is purified from goat anti-rabbit IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to rabbit IgG. Goat anti-rabbit is conjugated to ATTO 633 NHS (Abs.max. 629 nm; Em.max. 657 nm), (Cat. No. 01464), then further purified via gel permeation chromatography and dialysis to remove unbound ATTO-Dye.
Chemical Information
Product Specification
Application
| NACRES | NA.46 |
| Excitation | 636 |
| Emission | 650 |
| Properties Concentration | 1 mg/mL IgG |
| Properties Quality Level | 100 |
| Storage | −20 °C |
Anti-Rabbit IgG-ATTO 633 antibody, a fluorescently-labeled antibody produced in goat, is widely employed in biological research across various applications. Here are four key applications:
Immunofluorescence Microscopy: Utilizing Anti-Rabbit IgG-ATTO 633 in immunofluorescence microscopy enables the precise detection of specific antigens in fixed cells and tissues. The ATTO 633 dye boasts vibrant and enduring red fluorescence, facilitating high-resolution imaging. This application plays a pivotal role in visualizing the intricate localization and distribution patterns of target proteins across diverse biological samples, unraveling the spatial complexities of cellular structures.
Flow Cytometry: In the realm of flow cytometry, this antibody serves to quantify the presence of rabbit primary antibodies bound to their respective antigens on cell surfaces or within cells. The incorporation of a fluorescent tag allows for comprehensive multi-parameter analysis, offering detailed insights into the composition of cellular populations and protein expression levels. Researchers leverage this technique for immunophenotyping and functional assays, delving deep into the dynamic interplay of proteins within living systems.
Western Blotting: For Western blotting analyses, Anti-Rabbit IgG-ATTO 633 antibody emerges as a key player in detecting rabbit primary antibodies bound to target proteins after separation by SDS-PAGE and transfer onto membranes. The fluorescent ATTO 633 dye facilitates precise visualization of protein bands using state-of-the-art detection systems. This method serves as a cornerstone for validating protein expression levels, exploring post-translational modifications, and determining molecular weights with utmost accuracy.
Multiplexed Assays: The exceptional fluorescent attributes of ATTO 633 render this antibody ideal for multiplexed assays, where multiple targets are simultaneously detected in a single experiment. This capability proves especially valuable in high-throughput screening and diagnostic undertakings, where the concurrent analysis of multiple biomarkers is essential. The distinct fluorescence emission characteristics of ATTO 633 aid in clear differentiation from other fluorophores utilized in the assay, enabling efficient and reliable multiplexed analysis.
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