Anti-Mouse-IgG-ATTO 647N antibody produced in goat

Goat anti-Mouse IgG (H+L) antibody conjugated with ATTO 647N is an affinity-based fluorescent secondary antibody designed for robust fluorescence labeling of mouse primary antibodies in immunostaining workflows. The ATTO 647N fluorophore provides bright far-red emission that is commonly leveraged to reduce spectral overlap in multicolor imaging and to improve contrast in fluorescence microscopy and flow cytometry. This reagent is produced in goat and is intended for research-grade staining of mouse IgG targets, including antibody-based detection in cell biology, protein analysis, and biomarker workflows.

1. Fluorescence Microscopy Staining

Goat anti-Mouse IgG-ATTO 647N antibody produced in goat is widely used as a fluorescent secondary antibody to visualize mouse primary antibodies in fixed-cell and immunofluorescence microscopy experiments. Researchers use it to amplify the apparent signal of low-abundance mouse targets by binding the Fc/epitope regions of mouse IgG, enabling clear localization of proteins, transcription factors, and other antigen targets detected by mouse monoclonal or polyclonal primaries. The far-red ATTO 647N emission supports multichannel staining strategies where green and orange channels are already occupied, helping maintain separation between fluorophores during confocal and widefield imaging.

2. Flow Cytometry Secondary Labeling

Goat anti-Mouse IgG-ATTO 647N antibody produced in goat is used in flow cytometry workflows where mouse primary antibodies are followed by a fluorescent secondary to generate a measurable far-red signal on labeled cells. This format is common in immunophenotyping panels, antibody validation studies, and assay development for cell-surface or intracellular targets when direct conjugates are not available or when consistent secondary labeling is preferred across multiple mouse primaries. The ATTO 647N far-red spectral region is frequently chosen to facilitate panel design and to minimize spillover from fluorophores excited and detected in the visible range.

3. Western Blot Immunodetection

Goat anti-Mouse IgG-ATTO 647N antibody produced in goat supports fluorescence-based immunoblotting workflows by providing a far-red secondary readout for mouse IgG primary antibodies. In protein analysis settings, this reagent is used to detect bands corresponding to target proteins after transfer to membranes, offering a convenient fluorescence detection route that integrates well with modern imaging systems for blot documentation. The strong secondary-antibody binding to mouse IgG enables consistent visualization across replicate blots and supports multiplexing when compatible far-red channels are available.

4. Immunoassay And ELISA Readouts

Goat anti-Mouse IgG-ATTO 647N antibody produced in goat is also used in fluorescence-based immunoassays where mouse primary antibodies or mouse-derived capture reagents are followed by a fluorescent secondary for signal generation. Researchers apply it in assay development and antibody characterization to quantify binding events through fluorescence intensity measured on plate readers or imaging platforms. The ATTO 647N label provides a far-red reporting option that can be leveraged when assay formats require separation from other fluorescent reagents used in the same experiment.