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Product Introduction
ATTO 520 is a novel fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. Above pH 7.0 the dye exists in an equilibrium with a colourless form (pseudobase). This is a reversible reaction, which does not interfere with the process of coupling to proteins etc. On lowering the pH absorption and fluorescence are restored fully. For details of coupling see our recommended labeling procedures The fluorescence is excited most efficiently in the range 510-535 nm. A suitable source of excitation is an Argon-Ion laser using the 514 nm line.Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10-8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours. Fluorescent conjugates of phalloidin are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-d-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.find more information here
Chemical Information
Product Specification
Application
| NACRES | NA.32 |
| Excitation | 516 |
| Emission | 538 ±10 |
| Properties Quality Level | 100 |
| Storage | −20 °C |
Phalloidin-ATTO 520, a fluorescent conjugate widely employed for staining actin filaments in cellular and molecular biology, offers diverse applications. Here are four key applications:
Cell Imaging: In the realm of fluorescence microscopy, Phalloidin-ATTO 520 plays a pivotal role in visualizing actin filaments within both fixed and live cells. By specifically binding to F-actin, it enables researchers to explore the intricate cytoskeletal architecture and dynamics. This capability is paramount for unraveling the complexities of cellular processes such as motility, division, and shape maintenance.
Pathway Analysis: Delving into signaling pathways that involve actin reorganization, researchers leverage Phalloidin-ATTO 520 to shed light on critical cellular responses triggered by growth factors, hormones, and environmental stresses. By scrutinizing changes in actin patterns using this fluorescent probe, scientists can uncover the underlying molecular mechanisms orchestrating cellular behavior.
Drug Screening: In the realm of pharmaceutical research, Phalloidin-ATTO 520 finds utility in high-content screening assays aimed at evaluating the impact of drug candidates on the cytoskeleton. Compounds that target actin can induce significant alterations in cell morphology and function, making this technique invaluable for identifying potential therapeutic agents. This application streamlines drug discovery efforts and facilitates safety assessments of novel compounds.
Developmental Biology: Within the domain of developmental biology, Phalloidin-ATTO 520 serves as a valuable tool for studying the distribution and organization of actin during embryonic development. By visualizing actin structures in evolving tissues, researchers gain insights into processes such as morphogenesis, tissue remodeling, and organogenesis. This knowledge is instrumental in unraveling the intricacies of developmental pathways and pinpointing factors contributing to congenital disorders.
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