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Product Introduction
ATTO 665 is a new fluorescent label closely related to ATTO 647N. It shows extraordinary high fluorescence quantum yield, excellent thermal and photo-stability, outstanding ozone resistance, and very little triplet formation. ATTO 665 is a cationic (charge +1). The dye is moderately hydrophilic. The maleimide of the dye is suitable for labeling sulfhydryl (thiol) groups of proteins, in particular. Cystein residues. Prepare labeling solution of maleimide-derivative immediately before use by dissolving the vial content in anhydrous and amine-free DMF or DMSO.find more information here
Chemical Information
Application
| Purity | ≥90% (coupling to thiols) |
| NACRES | NA.32 |
ATTO 665 maleimide, a versatile fluorescent dye, finds widespread utility in labeling proteins, peptides, and other biomolecules across diverse scientific fields. Explore its key applications:
Fluorescence Microscopy: Delving into the realm of fluorescence microscopy, ATTO 665 maleimide emerges as a cornerstone for illuminating proteins and other cellular constituents. This luminous dye offers a window into molecular interactions and cellular architectures with unparalleled precision. Researchers delve into the vibrant dynamics of labeled molecules within live or fixed cells.
Flow Cytometry: In the intricate landscape of flow cytometry, ATTO 665 maleimide takes center stage as a beacon for labeling antibodies and ligands to detect and quantify cell surface markers. The radiance of ATTO 665 facilitates meticulous analysis of cell populations, crucial for immunophenotyping, cell sorting, and probing cellular responses at a granular level.
Fluorescence Resonance Energy Transfer (FRET) Studies: Embracing the complexity of FRET assays, ATTO 665 maleimide shines brightly with its exceptional spectral attributes. Paired with a compatible donor dye, it unravels the intricacies of protein-protein interactions and real-time conformational changes.
Protein Labeling for Biochemical Studies: Peering into the realm of biochemical investigations, ATTO 665 maleimide emerges as a potent tool for targeting specific cysteine residues on proteins, catalyzing a diverse array of biochemical analyses. Labeled proteins serve as linchpins in binding assays, structural explorations, and enzyme kinetics, unraveling the nuanced tapestry of protein functionality and interplay at a microscopic level.
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