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Product Introduction
ATTO Oxa12 maleimide ester is a red-emitting dye and a lipophilic variety of ATTO 655. Characteristic features of the label are strong absorption, good fluorescence quantum yield, very little triplet formation, excellent thermal and photo-stability, and outstanding ozone resistance.ATTO Oxa12 maleimide is a cationic dye (charge +1). Absorption and fluorescence are pH independent in the range pH 2 to 11, used in typical applications. ATTO Oxa12 maleimide shows excellent solubility in organic solvents like DMF, DMSO or acetonitrile.find more information here
Chemical Information
Application
| NACRES | NA.32 |
ATTO Oxa12 maleimide, a renowned fluorescent dye in the realm of bioscience, finds diverse applications. Here are four key uses of ATTO Oxa12 maleimide:
Fluorescent Labeling: Widely embraced for its maleimide functionality, ATTO Oxa12 maleimide serves as a go-to choice for labeling proteins and peptides. Its selective reactivity with thiol groups enables researchers to monitor and visualize biomolecules in live-cell imaging and flow cytometry settings. The dye’s remarkable photostability and brightness qualities render it an ideal candidate for in-depth and prolonged imaging endeavors.
Immunofluorescence: In the realm of immunofluorescence assays, ATTO Oxa12 maleimide plays a pivotal role in illuminating specific antigens within cells or tissue sections. By conjugating the dye to antibodies, scientists can precisely pinpoint and highlight cellular components under the lens of a fluorescence microscope. This technique is essential for scrutinizing the spatial distribution of proteins and discerning intricate cellular structures.
FRET Studies: At the forefront of scientific exploration, ATTO Oxa12 maleimide finds application in Förster Resonance Energy Transfer (FRET) investigations, delving into molecular interactions and distances between biomolecules. Through strategic pairing with a suitable FRET acceptor, researchers can track real-time conformational shifts and binding events at the molecular scale. This utilization is critical for unraveling the nuances of protein-protein interactions and dynamic cellular processes.
Bioconjugation: Within the domain of bioconjugation, ATTO Oxa12 maleimide emerges as a key player, facilitating the attachment of fluorescent labels to a diverse array of biomolecules, including oligonucleotides and small compounds. This capability empowers the exploration of molecular dynamics and interactions within complex biological systems. Its specific and enduring conjugation properties position it as the preferred choice for crafting tailored fluorescent probes tailored for cutting-edge research pursuits.
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