Anti-Rabbit-IgG-ATTO 550 antibody produced in goat

Goat anti-Rabbit IgG (H+L) conjugated to ATTO 550 is a fluorescent secondary antibody designed for immunofluorescence workflows where rabbit primary antibodies are used. The ATTO 550 fluorophore provides bright orange-red emission for microscopy and fluorescence-based assays, while the goat antibody format supports robust recognition of rabbit IgG. This reagent is commonly selected to generate consistent labeling of rabbit targets across fixed-cell imaging, Western blot immunostaining, and fluorescence readouts.

1. Immunofluorescence Microscopy

Goat anti-Rabbit IgG (H+L) conjugated to ATTO 550 is frequently used as a secondary antibody in fluorescence microscopy to visualize rabbit primary antibody-labeled antigens in fixed cells and tissue sections. Researchers and imaging core facilities rely on the secondary format to amplify signal by binding to rabbit IgG, enabling clear spatial localization of target proteins with an ATTO 550 emission channel compatible with standard fluorescence microscope filter sets. The conjugate format helps streamline staining workflows for multicolor experiments where an ATTO 550-based channel is required.

2. Flow Cytometry Labeling

Goat anti-Rabbit IgG (H+L) conjugated to ATTO 550 is applied in flow cytometry to detect rabbit antibody binding on permeabilized or fixed cell preparations, supporting fluorescence-based quantification of antigen-associated staining. In cell analysis workflows, the ATTO 550 conjugate is used to convert rabbit primary antibody binding into a measurable fluorescence signal, allowing researchers to compare staining intensity across experimental conditions. This secondary antibody format is particularly useful when rabbit primaries are already established in a panel and a consistent fluorescent readout is needed for gating and population analysis.

3. Western Blot Immunostaining

Goat anti-Rabbit IgG (H+L) conjugated to ATTO 550 is commonly used for fluorescence Western blot workflows to visualize rabbit primary antibody-recognized proteins on membranes. In protein analysis pipelines, the ATTO 550 secondary antibody provides a convenient fluorescent reporter that can be imaged using fluorescence detection systems, supporting band visualization without relying on enzyme-based color development. Laboratories often choose this conjugate to maintain workflow consistency across blots and to integrate immunostaining into imaging-based quantification strategies.

4. Fluorescent Multiplex Assays

Goat anti-Rabbit IgG (H+L) conjugated to ATTO 550 is also used in fluorescence multiplexing strategies where rabbit primary antibodies must be detected with a defined spectral channel. In assay development and biomarker research, the ATTO 550 conjugate helps assign rabbit-target staining to an orange-red detection window while other primaries are labeled with spectrally distinct secondaries. This approach supports structured panel design in immunostaining experiments and enables researchers to map multiple targets within the same sample while maintaining clear channel separation.