ATTO 488 amine

ATTO 488 amine is a fluorescent dye of the ATTO series, recognized for its high fluorescence quantum yield and excellent photostability. The amine variant of ATTO 488 is particularly valuable due to the presence of a reactive primary amine group. This chemical functionality allows it to be easily conjugated to various biomolecules, such as proteins or nucleic acids, thus enabling diverse biological labeling applications. By absorbing light at a wavelength of around 500 nm and emitting at around 520 nm, ATTO 488 amine serves as an effective tool in fluorescence microscopy, flow cytometry, and other bioimaging techniques, providing researchers with bright and stable fluorescent signals for accurate biological observations.

One of the primary applications of ATTO 488 amine is in fluorescence microscopy, a technique critical for visualizing cellular structures and biomolecular interactions at high resolution. The dye’s strong fluorescence and photostability make it an ideal candidate for live cell imaging. Researchers can conjugate ATTO 488 amine to antibodies or other targeting molecules, enabling the specific labeling of cellular components such as proteins, membranes, or nucleic acids. This allows scientists to explore dynamic processes within cells in real time, enhancing our understanding of cellular function and disease pathology.

ATTO 488 amine is also widely utilized in flow cytometry, a technique used to analyze the physical and chemical characteristics of particles as they flow in a fluid stream. By tagging cells or particles with ATTO 488 amine-conjugated antibodies, researchers can quantify and sort different cell populations based on specific markers. The high sensitivity and resolution provided by ATTO 488 amine facilitate detailed phenotypic analyses of complex samples, contributing to advancements in immunology, cancer research, and stem cell studies.

Another significant application is in the development of molecular probes for bioimaging. ATTO 488 amine can be conjugated to small molecules or nanoparticles that target specific cellular environments or biomolecules. These probes can then be used to visualize and track various biochemical processes within living organisms, offering insights into metabolic pathways, drug delivery efficacy, and cellular responses to external stimuli. Such applications are pivotal in the fields of pharmacology and medical diagnostics, where understanding molecular interactions can lead to improved therapeutics.

Lastly, ATTO 488 amine is employed in the field of protein and nucleic acid labeling. Bioconjugation techniques utilizing the amine group allow for the tagging of peptides, proteins, oligonucleotides, or whole nucleic acid strands with the fluorescent dye. This labeling is crucial in assays like fluorescence in situ hybridization (FISH) and enzyme-linked immunosorbent assays (ELISA), where it enables the detection and quantification of specific DNA, RNA, or protein targets. Through such biochemical assays, ATTO 488 amine aids in elucidating molecular mechanisms and streamlining diagnostic procedures, underscoring its versatility and importance in molecular biology research.