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Product Introduction
ATTO MB2-Biotin is a derivative of the well-known redox dye Methylene Blue. The dye can be reversibly reduced to the Colorless leuko form. Upon oxidation (e.g. with oxygen) the dye recovers, and the absorption is fully restored.The dye is suitable for labeling of DNA, RNA, proteins etc. In common with most ATTO-labels the dye shows a high extinction coefficient.ATTO MB2-Biotin ester is moderately hydrophilic.find more information here
Chemical Information
Application
| NACRES | NA.32 |
ATTO MB2-Biotin is a biotinylated fluorescent dye used in various applications within the bioscience field. Here are some key applications of ATTO MB2-Biotin:
Immunofluorescence: ATTO MB2-Biotin can be used to label antibodies for immunofluorescence microscopy. By conjugating the dye to secondary antibodies, researchers can visualize specific antigens in cell or tissue samples with high sensitivity. This enhances the ability to study protein localization, expression patterns, and cellular dynamics.
Flow Cytometry: In flow cytometry, ATTO MB2-Biotin is frequently used to stain cells in order to analyze populations based on specific markers. When combined with streptavidin, the biotin-labeled dye allows for multiplexing and detailed phenotypic analysis. This helps in assessing cellular composition, identifying rare cell types, and conducting high-throughput screening.
Protein-Protein Interaction Studies: ATTO MB2-Biotin is utilized in assays to study protein-protein interactions, such as pull-down assays and affinity binding assays. By incorporating the dye into one of the interacting partners, it becomes possible to track and measure binding events. This facilitates the identification of interaction partners and the characterization of binding affinities.
Live-Cell Imaging: This biotinylated fluorescent dye is also employed in live-cell imaging to monitor dynamic processes in living cells. By tagging molecules of interest, researchers can study real-time changes in cellular events, such as signaling, trafficking, and protein interactions. This contributes to a deeper understanding of cellular mechanisms and real-time biological responses.
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