Anti-Rabbit-IgG-ATTO 647N antibody produced in goat

Anti-Rabbit-IgG-ATTO 647N antibody produced in goat is a fluorescent secondary antibody reagent designed for immunofluorescence and fluorescence-based protein detection workflows. The goat anti-rabbit IgG format provides strong compatibility with rabbit primary antibodies, while the ATTO 647N fluorophore enables far-red imaging that is commonly used to reduce spectral overlap in multicolor microscopy and fluorescence assays. This conjugated antibody is typically used as a labeling reagent for fixed-cell staining, immunoblot visualization, and fluorescence readouts where robust far-red signal is advantageous.

1. Immunofluorescence Microscopy

Anti-Rabbit-IgG-ATTO 647N antibody produced in goat is widely used by cell biology and microscopy laboratories as a far-red secondary antibody for visualizing rabbit primary antibody targets in fixed specimens. Researchers employ it to generate bright, spatially resolved fluorescence for subcellular protein localization studies, including co-staining workflows where far-red emission helps separate channels from green and orange dyes. The conjugate format supports direct labeling of rabbit IgG-bound primary antibodies without requiring additional dye conjugation steps, streamlining routine staining of cultured cells, tissue sections, and immobilized samples.

2. Western Blot Fluorescent Detection

Anti-Rabbit-IgG-ATTO 647N antibody produced in goat is used in fluorescence western blot workflows to detect rabbit primary antibodies with far-red readout. Protein biochemistry groups and core facilities often choose ATTO 647N secondary labeling to enable sensitive imaging on appropriate fluorescence imaging systems while maintaining compatibility with multicolor detection strategies. In these applications, the antibody serves as a conjugated detection reagent that binds to rabbit IgG on membranes, supporting workflow consistency across repeated blotting experiments.

3. Flow Cytometry Antibody Staining

Anti-Rabbit-IgG-ATTO 647N antibody produced in goat can be applied as a fluorescent secondary antibody for flow cytometry-based staining when rabbit primary antibodies are used to label intracellular or surface antigens. Flow cytometry users rely on the far-red fluorophore to help manage spectral separation in multiplex panels, particularly when other fluorophores occupy the green/orange regions. The conjugated antibody format supports direct fluorescence readout of rabbit primary binding events, enabling quantitative comparison of staining intensity across experimental conditions.

4. Multicolor Immunostaining Panels

Anti-Rabbit-IgG-ATTO 647N antibody produced in goat is frequently incorporated into multicolor immunostaining designs where far-red emission is used to reduce cross-talk between fluorescent channels. Imaging and assay development teams use this secondary antibody to pair rabbit primary targets with other labeled antibodies or stains, supporting coordinated localization studies and panel-based characterization of protein expression patterns. Its secondary-antibody role helps standardize labeling across targets while keeping the far-red channel dedicated to rabbit primary detection in complex staining schemes.