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Product Introduction
ATTO 550 is a fluorescent label related to the well-known dye Rhodamine 6G. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and very little triplet formation. The dye is highly suitable for single-molecule detection applications and high-resolution microscopy.ATTO 550 is a cationic dye (charge +1). As supplied ATTO 550 consists of three isomers with identical absorption and fluorescence.The alkyne modification is used in the Huisgen reaction (“Click Chemistry“).find more information here
Chemical Information
Application
| NACRES | NA.32 |
ATTO 550 alkyne, a fluorescent dye widely employed in diverse biological and chemical research applications, boasts a range of key uses. Here are four applications:
Fluorescence Microscopy: The utilization of ATTO 550 alkyne in fluorescence microscopy revolutionizes the labeling and visualization of cellular structures with exceptional specificity and resolution. By linking this dye to biomolecules like antibodies or peptides, researchers can meticulously monitor the whereabouts and dynamics of proteins within live or fixed cells. This technique facilitates real-time scrutiny of cellular processes under physiologically relevant conditions, offering unparalleled insights into cellular dynamics.
Flow Cytometry: An indispensable tool in flow cytometry, ATTO 550 alkyne facilitates the analysis and sorting of cells based on their fluorescence properties. When coupled with antibodies targeting specific cell surface markers, ATTO 550 alkyne enables the identification and quantification of diverse cell populations within a heterogeneous mixture. This capability is pivotal for immunophenotyping, stem cell research, and evaluating cellular responses in a nuanced and comprehensive manner.
Super-Resolution Imaging: Positioned at the forefront of super-resolution imaging methodologies like STED (Stimulated Emission Depletion) microscopy and PALM (Photo-Activated Localization Microscopy), ATTO 550 alkyne excels in visualizing structures at the nanometer scale, surpassing the diffraction limit of conventional microscopy. The remarkable photostability and brightness of ATTO 550 alkyne render it the perfect candidate for capturing intricate images of subcellular components with unparalleled clarity and precision.
Bioconjugation and Click Chemistry: Serving as a key player in bioorthogonal click chemistry, ATTO 550 alkyne facilitates the specific and covalent attachment of the dye to biomolecules in a controlled fashion. The alkyne group engages in copper-catalyzed click reactions with azide-modified targets, ensuring precise and targeted labeling. This methodology finds extensive use in tagging proteins, nucleic acids, and lipids, enabling a diverse array of applications in molecular biology and biotechnology.
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