NH-bis(C2-PEG1-azide) | CAS 2100306-81-6

NH-bis(C2-PEG1-azide) is a bis-azide, PEG-based click chemistry reagent designed for copper-catalyzed azide–alkyne cycloaddition (CuAAC) workflows. The scaffold presents two azide handles on a flexible polyethylene glycol framework, enabling multivalent conjugation and modular assembly of functional biomolecules, probes, and materials. Its PEG architecture is commonly leveraged to improve solubility and spacing between reactive groups in labeling, surface functionalization, and polymer conjugation strategies.

1. Multivalent Biomolecule Labeling

NH-bis(C2-PEG1-azide) is used to introduce two orthogonally addressable azide sites for multivalent labeling of proteins, peptides, and other biomolecular platforms that are subsequently decorated with alkyne-bearing partners via CuAAC. Researchers select this reagent when a single labeling event is insufficient to achieve the desired probe density or when improved hydrophilicity and reduced aggregation are needed for downstream imaging, binding assays, or platform compatibility. The PEG spacing supports more uniform conjugate formation and can help maintain accessibility of functional groups on large or crowded biomolecular constructs.

2. Surface and Hydrogel Functionalization

NH-bis(C2-PEG1-azide) is applied in the fabrication of azide-functional surfaces and PEG-containing hydrogels where subsequent CuAAC coupling to alkyne-modified linkers, affinity ligands, or reporter moieties is required. Materials scientists and biomaterials teams use bis-azide reagents to tune crosslinking density, immobilize bioactive components, and create spatially controlled functional layers on substrates such as glass, polymers, and biointerfaces. The dual azide functionality supports multivalent attachment schemes that are valuable for building stable, high-capacity material coatings and for generating reproducible reagent-to-surface coupling in research-grade workflows.

3. Molecular Imaging Probe Assembly

NH-bis(C2-PEG1-azide) supports the modular construction of imaging reagents by providing a robust azide motif for CuAAC attachment of alkyne-tagged fluorophores, luminescent reporters, or imaging-compatible cofactors. Molecular imaging and chemical biology groups often choose bis-azide PEG scaffolds to manage labeling stoichiometry and to increase effective probe loading through multivalent conjugation strategies. By using NH-bis(C2-PEG1-azide) as a flexible linker, teams can assemble conjugates with improved aqueous handling and controlled presentation of imaging components for standardized probe development.

4. Polymer and Nanomaterial Conjugation

NH-bis(C2-PEG1-azide) is widely used in polymer chemistry and nanomaterials research to create azide-functional macromolecular architectures that can be post-functionalized with alkyne-bearing building blocks. Industrial and academic laboratories employ the bis-azide PEG structure to generate multivalent coupling points for grafting, end-group modification, and surface-to-core or core-to-shell conjugation in colloidal systems. This approach is particularly useful when researchers need consistent spacing of reactive sites and when maintaining solubility and colloidal stability is important for reproducible materials processing and characterization.