N,N'-bis-(acid-PEG3)-Benzothiazole Cy5 | CAS 2107273-82-3

N,N'-bis-(acid-PEG3)-Benzothiazole Cy5 is a Cy5-class near-infrared fluorophore designed for conjugation-ready labeling, incorporating PEG3-acid handles to support aqueous compatibility and downstream attachment to biomolecules, peptides, and polymeric materials. The benzothiazole motif provides a strong Cy5-based fluorescence signal in the far-red region, making the reagent useful for fluorescence imaging workflows where reduced background from autofluorescence is advantageous.

1. Fluorescence Imaging Labeling

N,N'-bis-(acid-PEG3)-Benzothiazole Cy5 is used by imaging-focused chemistry and cell biology groups to generate fluorescently labeled biomolecule conjugates for fluorescence microscopy and optical imaging studies. Researchers commonly attach the Cy5 fluorophore to proteins, peptides, or other targeting ligands to visualize binding, trafficking, or distribution in labeled samples, leveraging the far-red emission to improve contrast against cellular autofluorescence. The PEG3-acid functionality supports conjugate construction in aqueous labeling workflows, enabling preparation of imaging reagents for instrument platforms that detect Cy5 emission.

2. Bioconjugation for Protein Tracing

N,N'-bis-(acid-PEG3)-Benzothiazole Cy5 supports fluorescence labeling of proteins and related bioconjugates used in biochemical characterization and molecular tracing experiments. In research settings, the reagent is incorporated into labeling strategies for tracking biomolecule interactions, monitoring localization in complex mixtures, and preparing fluorescent standards for comparative assays. The PEG3-acid design helps maintain conjugate processability during reagent preparation and downstream handling, which is particularly relevant when preparing multiple labeled constructs for imaging or assay development.

3. Nucleic Acid and Probe Conjugates

N,N'-bis-(acid-PEG3)-Benzothiazole Cy5 is frequently used in fluorescent probe development pipelines to generate Cy5-labeled oligonucleotide or nucleic-acid-associated conjugates for fluorescence readouts. Molecular biology and chemical biology teams use far-red Cy5 labeling to monitor hybridization-dependent signals, track labeled strands in analytical workflows, and build fluorescence-based detection reagents compatible with common fluorescence instrumentation. The conjugation-ready PEG3-acid functionality facilitates incorporation into nucleic-acid labeling formats where aqueous handling and stable reagent preparation are important.

4. Polymer and Biomaterial Fluorescent Tagging

N,N'-bis-(acid-PEG3)-Benzothiazole Cy5 is applied in biomaterials and materials chemistry to fluorescently tag polymers, hydrogels, and surface-modified constructs for localization and stability studies. Researchers incorporate the Cy5 fluorophore into material systems to visualize distribution, coating uniformity, and retention of fluorescent labels in complex matrices. The PEG3-acid handles are particularly useful for preparing labeled materials that can be processed in aqueous or mixed-solvent environments, supporting fluorescence-based characterization with far-red detection.