N-(m-PEG4)-N'-(hydroxy-PEG2)-Cy5 | 2107273-12-9

N-(m-PEG4)-N’-(hydroxy-PEG2)-Cy5, a versatile fluorescent dye frequently utilized in biological research, boasts a multitude of applications. Here are four key applications:

Fluorescence Microscopy: An indispensable tool in modern research, N-(m-PEG4)-N’-(hydroxy-PEG2)-Cy5 finds common usage in fluorescence microscopy for labeling and visualizing cellular constituents. With its bright and enduring fluorescence properties, this dye is an optimal choice for conducting extensive imaging studies over prolonged durations. Researchers can delve into the molecular intricacies and interactions within cells at a microscopic level, unraveling the mysteries of cellular dynamics.

Flow Cytometry: Embracing the capabilities of N-(m-PEG4)-N’-(hydroxy-PEG2)-Cy5, scientists apply this dye in flow cytometry to tag cells and scrutinize their attributes, such as size, complexity, and specific markers. Through multiplexing, researchers can concurrently detect a myriad of parameters within a single cell, enhancing the ability to differentiate distinct cell populations and their functional statuses. This versatile dye elevates the resolution and depth of insights gleaned from flow cytometric analyses.

Protein Labeling: Championing the realm of protein studies, N-(m-PEG4)-N’-(hydroxy-PEG2)-Cy5 serves as a valuable asset for conjugating with proteins to investigate their spatial distribution, binding affinities, and intracellular trafficking patterns. By affixing proteins with this vibrant fluorescent tag, researchers can conduct quantitative assessments utilizing diverse fluorescence-based methodologies. This strategic approach facilitates real-time exploration of protein functionalities and interactions, shedding light on the intricate web of protein dynamics.

In Vivo Imaging: Transcending boundaries, this fluorescent marvel finds application in in vivo imaging endeavors to monitor biological processes within living organisms. Harnessing its near-infrared fluorescence prowess, N-(m-PEG4)-N’-(hydroxy-PEG2)-Cy5 enables deep tissue penetration while mitigating background interferences. Researchers leverage this dye to meticulously observe disease advancements, track drug delivery pathways, and evaluate therapeutic responses in a non-invasive manner.