N-(m-PEG9)-N'-(propargyl-PEG9)-Cy5
Catalog Number | A17-0158 |
Category | Cyanine5 |
Molecular Formula | C65H103ClN2O18 |
Molecular Weight | 1236.0 |
Catalog Number | Size | Price | Quantity |
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A17-0158 | -- | $-- |
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Product Introduction
N-(m-PEG9)-N'-(propargyl-PEG8)-Cy5 is a cyanine linker with excitation/emmission maximum 649/667 nm containing an alkyne group, which enables Click Chemistry to attach deeply colored and photostable Cyanine fluorophore to various molecules.
Chemical Information |
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Purity | 97% |
IUPAC Name | (2E)-2-[(2E,4E)-5-[3,3-dimethyl-1-[2-[2-[2-[2-[2-[2-[2-[2-(2-prop-2-ynoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethyl]indol-1-ium-2-yl]penta-2,4-dienylidene]-1-[2-[2-[2-[2-[2-[2-[2-[2-(2-methoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethyl]-3,3-dimethylindole;chloride |
Canonical SMILES | CC1(C2=CC=CC=C2[N+](=C1C=CC=CC=C3C(C4=CC=CC=C4N3CCOCCOCCOCCOCCOCCOCCOCCOCCOC)(C)C)CCOCCOCCOCCOCCOCCOCCOCCOCCOCC#C)C.[Cl-] |
InChI | InChI=1S/C65H103N2O18.ClH/c1-7-23-69-28-31-73-36-39-77-44-47-81-52-55-85-57-54-83-49-46-79-41-38-75-33-30-71-25-22-67-61-18-14-12-16-59(61)65(4,5)63(67)20-10-8-9-19-62-64(2,3)58-15-11-13-17-60(58)66(62)21-24-70-29-32-74-37-40-78-45-48-82-53-56-84-51-50-80-43-42-76-35-34-72-27-26-68-6;/h1,8-20H,21-57H2,2-6H3;1H/q+1;/p-1 |
InChI Key | JZVAYHBQJGOEPM-UHFFFAOYSA-M |
Solubility | Water, DMSO, DMF, DCM |
- Product Specification
- Application
Excitation | 649 |
Emission | 667 |
Storage | -20 °C |
N-(m-PEG9)-N’-(propargyl-PEG9)-Cy5, a bichromophoric compound renowned for its fluorescence properties, finds extensive utility in various biochemical applications. Here are four key applications of N-(m-PEG9)-N’-(propargyl-PEG9)-Cy5:
Fluorescence Imaging: Positioned at the forefront of fluorescence microscopy and imaging technologies, N-(m-PEG9)-N’-(propargyl-PEG9)-Cy5 serves as a beacon for visualizing dynamic biological processes in real-time. Its robust fluorescence illuminates specific cellular structures, proteins, or nucleic acids, facilitating intricate imaging studies. This compound plays a pivotal role in both live-cell imaging and fixed-cell staining protocols.
Protein Labeling: In the realm of biochemical exploration, N-(m-PEG9)-N’-(propargyl-PEG9)-Cy5 emerges as a versatile tool for protein labeling, enabling a myriad of analytical applications. The propargyl group’s incorporation allows for bioorthogonal labeling via click chemistry, turning it into a dynamic asset for studying protein dynamics, interactions, and localization.
Flow Cytometry: Harnessing the vibrant fluorescence of N-(m-PEG9)-N’-(propargyl-PEG9)-Cy5, flow cytometry delves into the realm of cell analysis and sorting with unprecedented precision. By leveraging the compound’s intense fluorescence, researchers can dissect cell populations based on specific biomarker expressions, enhancing the resolution and sensitivity of cellular analyses. This application assumes critical importance in fields such as immunology, cancer research, and stem cell investigations.
Computed Properties | |
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Hydrogen Bond Donor Count | 0 |
Hydrogen Bond Acceptor Count | 20 |
Rotatable Bond Count | 58 |
Exact Mass | 1234.6894421 g/mol |
Monoisotopic Mass | 1234.6894421 g/mol |
Topological Polar Surface Area | 172Ų |
Heavy Atom Count | 86 |
Formal Charge | 0 |
Complexity | 1790 |
Isotope Atom Count | 0 |
Defined Atom Stereocenter Count | 0 |
Undefined Atom Stereocenter Count | 0 |
Defined Bond Stereocenter Count | 3 |
Undefined Bond Stereocenter Count | 0 |
Covalently-Bonded Unit Count | 2 |
Compound Is Canonicalized | Yes |
Applications of Fluorescent Probes & Dyes
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