N-methyl-N'-methyl-O-(m-PEG4)-O'-(azide-PEG4)-Cy5 | CAS 2107273-56-1

N-methyl-N’-methyl-O-(m-PEG4)-O’-(azide-PEG4)-Cy5, a prominent fluorescent compound in biochemical research and diagnostics, finds diverse applications. Here are four key uses:

Fluorescence Imaging: Delving into the intricate realm of fluorescence imaging, this compound serves as a powerful tool for visualizing and tracking biological processes at cellular and molecular scales. Upon conjugation with specific biomolecules, it enables in-depth examination of protein dynamics, nucleic acid localization, and other cellular components, unraveling nuanced insights into cellular functions and aberrations.

Flow Cytometry: In the domain of flow cytometry, N-methyl-N’-methyl-O-(m-PEG4)-O’-(azide-PEG4)-Cy5 plays a pivotal role in analyzing cell populations with precision. By tagging cells with this fluorescent marker, researchers can quantitatively assess and segregate cells based on distinct characteristics such as protein expression levels or cell cycle statuses. This technique is indispensable for advancing fields like immunology, cancer research, and stem cell biology.

Bioconjugation: Featuring an azide group conducive to bioorthogonal conjugation through click chemistry, this compound facilitates targeted attachment of the Cy5 fluorophore to diverse biomolecules. This unique property enables site-specific labeling of proteins, peptides, and nucleic acids, pivotal for probing molecular interactions and devising cutting-edge diagnostic assays.

Live-Cell Imaging: With its PEGylated structure enhancing solubility and biocompatibility, this compound emerges as an ideal candidate for live-cell imaging endeavors. Researchers leverage its capabilities to accurately label live cells without disrupting their normal functionalities, capturing real-time insights into dynamic cellular processes like signal transduction, migration, and cell division. This application stands as an essential tool for unraveling the complexities of cellular behavior.