N6-(6-Aminohexyl)-ATP-Cy5 Triethylammonium salt

N6-(6-Aminohexyl)-ATP-Cy5 Triethylammonium salt is a fluorescent nucleotide analog used in various biochemical and cell biology applications. Here are some key applications of N6-(6-Aminohexyl)-ATP-Cy5 Triethylammonium salt:

Fluorescent Tagging of Nucleotides: N6-(6-Aminohexyl)-ATP-Cy5 is employed to label nucleotides in biochemical assays, allowing researchers to visualize and track nucleotide incorporation. This application is particularly useful in studying DNA and RNA synthesis, as well as in nucleotide-binding protein interactions. The Cy5 fluorophore provides strong fluorescence, facilitating high-resolution imaging and quantitative analysis.

Enzyme Activity Assays: Researchers use N6-(6-Aminohexyl)-ATP-Cy5 to investigate the activity of ATP-dependent enzymes such as kinases and polymerases. By incorporating the labeled ATP into their reactions, they can monitor enzyme kinetics and substrate specificity through fluorescence-based detection methods. This helps in understanding enzyme mechanisms and screening for potential inhibitors.

Cellular Imaging: This fluorescent ATP analog can be used in live-cell imaging to study cellular processes that involve ATP. For instance, it can be introduced into cells to monitor ATP-utilizing processes like signal transduction, metabolism, and vesicle trafficking. The Cy5 fluorescence allows for real-time visualization of these dynamic cellular events.

Protein-ATP Interaction Studies: N6-(6-Aminohexyl)-ATP-Cy5 is valuable in studying the binding interactions between proteins and ATP. By using this labeled nucleotide in binding assays, researchers can identify and characterize ATP-binding proteins and their interaction dynamics. This information is critical for understanding various cellular functions and for drug development targeting ATP-utilizing proteins.