N-(m-PEG4)-N'-(m-PEG4)-O-(m-PEG4)-O'-(azide-PEG4)-Cy5 | CAS 2107273-58-3

Name: N-(m-PEG4)-N'-(m-PEG4)-O-(m-PEG4)-O'-(azide-PEG4)-Cy5
Brand: BOC Sciences
Rating: 5 (1 reviews)

Catalog Number	A17-0145
Category	Cyanine5
Molecular Formula	C₆₂H₁₀₀ClN₅O₁₈
Molecular Weight	1238.93

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Product Introduction

N-(m-PEG4)-N'-(m-PEG4)-O-(m-PEG4)-O'-(azide-PEG4)-Cy5 is a polyethylene glycol (PEG)-based PROTAC linker. N-(m-PEG4)-N'-(m-PEG4)-O-(m-PEG4)-O'-(azide-PEG4)-Cy5 can be used in the synthesis of a series of PROTACs.

Chemical Information
Product Specification
Application

Chemical Information

Purity	98%
IUPAC Name	2-[(1E,3E)-5-[5-[2-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethoxy]ethoxy]-1-[2-[2-[2-(2-methoxyethoxy)ethoxy]ethoxy]ethyl]-3,3-dimethylindol-2-ylidene]penta-1,3-dienyl]-5-[2-[2-[2-(2-methoxyethoxy)ethoxy]ethoxy]ethoxy]-1-[2-[2-[2-(2-methoxyethoxy)ethoxy]ethoxy]ethyl]-3,3-dimethylindol-1-ium;chloride
SMILES	CC1(C2=C(C=CC(=C2)OCCOCCOCCOCCOC)[N+](=C1C=CC=CC=C3C(C4=C(N3CCOCCOCCOCCOC)C=CC(=C4)OCCOCCOCCOCCOCCN=[N+]=[N-])(C)C)CCOCCOCCOCCOC)C.[Cl-]
InChI	InChI=1S/C62H100N5O18.ClH/c1-61(2)55-51-53(84-49-47-82-45-43-80-40-37-76-28-25-70-7)13-15-57(55)66(18-21-72-30-33-78-38-35-74-26-23-68-5)59(61)11-9-8-10-12-60-62(3,4)56-52-54(85-50-48-83-46-44-81-42-41-77-32-29-71-20-17-64-65-63)14-16-58(56)67(60)19-22-73-31-34-79-39-36-75-27-24-69-6;/h8-16,51-52H,17-50H2,1-7H3;1H/q+1;/p-1
InChIKey	PKHNSJLVAZPWTJ-UHFFFAOYSA-M
Solubility	Water, DMSO, DMF, DCM

Product Specification

Excitation	649
Emission	667
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.

Application

N-(m-PEG4)-N'-(m-PEG4)-O-(m-PEG4)-O'-(azide-PEG4)-Cy5 is a Cy5-based fluorescent labeling reagent bearing a terminal azide group for bioorthogonal click chemistry and multiple m-PEG4 spacers that improve aqueous compatibility and reduce nonspecific interactions. The Cy5 fluorophore enables red fluorescence readouts in fluorescence microscopy and flow cytometry workflows, while the azide handle allows modular conjugation to alkyne-functional biomolecules, targeting ligands, and polymer or surface platforms.

1. Biomolecule Labeling

N-(m-PEG4)-N'-(m-PEG4)-O-(m-PEG4)-O'-(azide-PEG4)-Cy5 is used to generate fluorescently labeled proteins, peptides, and affinity reagents via azide-alkyne click conjugation, supporting downstream imaging and quantification in chemical biology and assay development. Researchers commonly incorporate the PEG4 spacers to help maintain conjugate solubility and to moderate dye-biomolecule proximity effects that can otherwise influence brightness and background in complex biological matrices. Typical workflows include preparing Cy5-tagged binding reagents for binding studies, reagent characterization, and fluorescence-based tracking of biomolecular interactions.

2. Fluorescence Microscopy Tracing

N-(m-PEG4)-N'-(m-PEG4)-O-(m-PEG4)-O'-(azide-PEG4)-Cy5 supports cellular and biomaterial imaging experiments where red-channel fluorescence is advantageous for multicolor panels and spectral separation. In microscopy workflows, the azide functionality is leveraged to attach Cy5 to alkyne-bearing targeting vectors, extracellular matrix components, or polymeric carriers, enabling visualization of labeled constructs in fixed or prepared samples. The PEGylated architecture helps reduce aggregation and promotes more uniform staining of click-labeled materials, which is particularly useful when imaging heterogeneous samples such as cell-associated conjugates or surface-tethered assemblies.

3. Flow Cytometry Conjugates

N-(m-PEG4)-N'-(m-PEG4)-O-(m-PEG4)-O'-(azide-PEG4)-Cy5 is frequently employed to prepare Cy5 fluorescence conjugates for flow cytometry-based analysis of labeled biomolecules and engineered binding reagents. By using the terminal azide group in click labeling, users can generate Cy5-tagged constructs from alkyne-functional antibodies, ligands, or other affinity components while benefiting from PEG4 spacers that can help limit nonspecific adsorption during staining and wash steps. This reagent format is particularly useful for building reproducible labeling reagents for instrument-ready fluorescence measurements in research settings.

4. Surface And Hydrogel Functionalization

N-(m-PEG4)-N'-(m-PEG4)-O-(m-PEG4)-O'-(azide-PEG4)-Cy5 is used to introduce Cy5 fluorescence onto alkyne-functional surfaces, nanoparticles, and hydrogel materials through click-compatible coupling strategies. Materials scientists and biomaterials teams incorporate the azide-PEG4-Cy5 motif to create fluorescently traceable coatings and scaffolds for studying material distribution, retention, and assembly in microscopy and imaging assays. The PEG spacers help maintain the dye's accessibility and reduce steric constraints when tethered to polymer networks or surface chemistries, supporting consistent visualization of engineered constructs during experimental workflows.