N-(m-PEG4)-3,3-Dimethyl-3H-indole-N'-(acid-PEG3)-Benzothiazole Cy5 | CAS 2107273-86-7

N-(m-PEG4)-3,3-Dimethyl-3H-indole-N'-(acid-PEG3)-Benzothiazole Cy5 is a Cy5-based fluorescent conjugate designed for aqueous labeling workflows where PEGylation helps improve solubility and reduce nonspecific interactions. Its extended conjugation and Cy5 emission make it useful for fluorescence imaging and assay readouts that rely on robust far-red detection, while the PEG handles support downstream conjugation or incorporation into larger probe constructs. Researchers commonly use this dye in fluorescence labeling and molecular imaging reagent development for labeling biomolecular carriers, affinity reagents, and PEGylated materials.

1. Fluorescence Imaging Labeling

N-(m-PEG4)-3,3-Dimethyl-3H-indole-N'-(acid-PEG3)-Benzothiazole Cy5 is used as a far-red fluorescence label in fluorescence microscopy and molecular imaging studies where reduced background and strong Cy5 emission are advantageous. In practice, teams incorporate the dye into labeled biomolecular constructs and imaging reagents to visualize distribution in labeled samples, including labeled polymeric carriers and PEG-functional materials. The PEG architecture supports compatibility with aqueous buffers commonly used for imaging experiments, making it a practical choice for building imaging probes that require stable dye presentation on larger scaffolds.

2. Bioconjugation Probe Construction

N-(m-PEG4)-3,3-Dimethyl-3H-indole-N'-(acid-PEG3)-Benzothiazole Cy5 is frequently selected for fluorescence probe development where PEGylated dye scaffolds are incorporated into bioconjugates for targeted or modular labeling strategies. Chemical biology and bioconjugation teams use the dye as a fluorescent reporter on larger constructs such as labeled affinity reagents, PEGylated linkers, and imaging-compatible probe platforms. The acid-PEG3 functionality and PEG spacing help support reliable attachment strategies during probe assembly, enabling researchers to generate conjugates for fluorescence-based tracking and localization studies in complex biological matrices.

3. Flow Cytometry Fluorescent Reporter

N-(m-PEG4)-3,3-Dimethyl-3H-indole-N'-(acid-PEG3)-Benzothiazole Cy5 is applied as a far-red flow cytometry reporter dye in workflows that require Cy5-channel readout for labeling and population analysis. In typical use, researchers conjugate the dye to targeting or binding components and then quantify fluorescence in cell suspensions by flow cytometry to monitor labeling intensity and distribution across experimental conditions. The PEGylated dye format supports consistent performance in aqueous staining buffers, supporting reproducible staining and readout during assay development and screening experiments.

4. Fluorescence-Based Bioassay Reagents

N-(m-PEG4)-3,3-Dimethyl-3H-indole-N'-(acid-PEG3)-Benzothiazole Cy5 is used in fluorescence assay reagent development where far-red fluorescence enables multiplex-friendly detection and minimizes spectral overlap with shorter-wavelength labels. Assay developers incorporate the dye into labeled assay components to generate fluorescence readouts for binding and interaction studies, including platform validation for fluorescence-based screening. The dye's Cy5 emission supports sensitive signal collection on standard fluorescence plate readers and imaging systems configured for far-red channels, helping teams build assay-ready fluorescent reagents for research use.