LumiCell CDr20 Microglia Stain | CAS 1201643-01-7

LumiCell CDr20 Microglia Stain is a research fluorescence staining reagent designed to label microglia-associated targets for cellular imaging workflows. The stain provides a convenient, microscopy-ready signal that supports visualization of microglia in complex biological preparations, including co-culture and tissue-derived samples, where cell-type discrimination is required. Its fluorescence readout is intended for standard fluorescence imaging and quantitative imaging analysis, enabling researchers to track microglia localization and relative abundance under experimental conditions.

1. Microglia Fluorescence Microscopy

LumiCell CDr20 Microglia Stain is used in fluorescence microscopy to visualize microglia populations with a bright, stain-derived signal that supports cell-type-specific imaging in primary cultures and prepared samples. Researchers commonly apply the reagent during immunostaining-style workflows to generate microglia maps within mixed cell environments, such as neuron-glia co-cultures or glial cultures, where morphological context and spatial distribution are important. The resulting stained cells are compatible with routine fluorescence imaging pipelines used for qualitative assessment and quantitative image analysis of microglia coverage and distribution.

2. Co-Culture Cell-Type Discrimination

LumiCell CDr20 Microglia Stain is frequently incorporated into co-culture studies to distinguish microglia from neighboring cell types during experimental phenotyping. In mixed cultures, the stain helps researchers assign fluorescence signal to microglia-associated cells while other markers can be used in parallel for nuclei, astrocytes, or neuronal structures. This workflow supports comparative studies of microglia behavior across treatment conditions by enabling consistent visualization of microglia morphology and relative abundance within the same field of view.

3. Tissue-Derived Sample Staining

LumiCell CDr20 Microglia Stain is applied in prepared tissue or tissue-derived preparations to support microglia visualization in situ-like contexts for microscopy-based studies. Investigators use the reagent to label microglia-associated cells within complex backgrounds, enabling region-specific assessment of microglia presence and spatial organization. The stain is particularly useful when researchers need a fluorescence signal that integrates into established staining and imaging routines used for mapping cell populations across sample sections or prepared tissue matrices.

4. Imaging-Based Quantification Workflows

LumiCell CDr20 Microglia Stain supports imaging-based quantification by providing a consistent fluorescent label that can be analyzed using common image processing tools. Researchers use the stained microglia signal to quantify relative cell area, cell counts, or spatial distribution metrics across experimental groups, often pairing the stain with standardized imaging settings to improve comparability across batches. This makes the reagent a practical choice for assay development and routine microscopy studies where reproducible fluorescence labeling is required for downstream quantitative analysis.