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Z-LLE-AMC | CAS 348086-66-8
| Catalog Number | A18-0062 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C35H44N4O9 |
| Molecular Weight | 664.76 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Z-LLE-AMC is a fluorogenic substrate for the caspase-like post-glutamate peptide hydrolase of the 26S proteasome or 20S proteolytic core. AMC (7-amino-4-methylcoumarin) is released to quantify the caspase-like hydrolase activity.
Chemical Information
Product Specification
Application
| Synonyms | Proteasome Substrate III, Fluorogenic |
| Purity | >95% |
| IUPAC Name | (4S)-4-[[(2S)-4-methyl-2-[[(2S)-4-methyl-2-(phenylmethoxycarbonylamino)pentanoyl]amino]pentanoyl]amino]-5-[(4-methyl-2-oxochromen-7-yl)amino]-5-oxopentanoic acid |
| Canonical SMILES | CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CCC(=O)O)NC(=O)C(CC(C)C)NC(=O)C(CC(C)C)NC(=O)OCC3=CC=CC=C3 |
| InChI | InChI=1S/C35H44N4O9/c1-20(2)15-27(38-34(45)28(16-21(3)4)39-35(46)47-19-23-9-7-6-8-10-23)33(44)37-26(13-14-30(40)41)32(43)36-24-11-12-25-22(5)17-31(42)48-29(25)18-24/h6-12,17-18,20-21,26-28H,13-16,19H2,1-5H3,(H,36,43)(H,37,44)(H,38,45)(H,39,46)(H,40,41)/t26-,27-,28-/m0/s1 |
| InChIKey | FOYHOBVZPWIGJM-KCHLEUMXSA-N |
| Solubility | Soluble in DMSO |
| Density | 1.253±0.06 g/cm3(Predicted) |
| Appearance | Solid Lyophilized Powder |
| Boiling Point | 967.8±65.0°C at 760 mmHg |
| Excitation | 340-360 nm |
| Emission | 440-460 nm |
| Storage | Store at -20°C |
Z-LLE-AMC is a synthetic peptide substrate used in various biochemical and cellular assays. Here are some key applications of Z-LLE-AMC:
Protease Activity Assays: Z-LLE-AMC is widely used to measure the activity of proteolytic enzymes, particularly those belonging to the class of cysteine proteases. By monitoring the fluorescence release upon cleavage of the peptide, researchers can quantify enzyme activity in real-time. This application is essential for studying enzyme kinetics and screening potential inhibitors.
Cell Biology Research: Z-LLE-AMC can be utilized in cellular assays to investigate protease activity within live cells. The substrate is internalized by cells, where it is cleaved by intracellular proteases, leading to a fluorescent signal that can be quantified. This approach helps in understanding protease functions in cellular processes such as apoptosis, autophagy, and protein degradation.
Drug Development: In the pharmaceutical industry, Z-LLE-AMC serves as a tool for screening and profiling protease inhibitors. By providing a reliable readout of protease activity, it enables the identification of compounds that can modulate enzyme function. This is critical for developing new drugs targeting protease-related diseases such as cancer and neurodegenerative disorders.
Biotechnology: Z-LLE-AMC is used in biotechnological applications to monitor proteolytic processes in various systems, including fermentation and bioreactor operations. By tracking protease activity, researchers can optimize conditions for recombinant protein production and ensure product quality. This substrate aids in the efficient monitoring and control of bioprocesses.
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