4-Methylumbelliferyl-β-D-Glucuronide hydrate | CAS 881005-91-0

Catalog Number	A18-0016
Category	Fluorescent Enzyme Substrates
Molecular Formula	C16H16O9.xH2O
Molecular Weight	352.29 (anhydrous basis)

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Product Introduction

4-Methylumbelliferyl-β-D-glucuronide is a fluorogenic substrate for β-glucuronidase. 4-Methylumbelliferyl-β-D-glucuronide is cleaved by β-glucuronidase to release the fluorescent moiety 4-methylumbelliferyl (4-MU).

Chemical Information
Product Specification
Application

Related CAS	1049741-34-5 (Deleted CAS) 6160-80-1 (anhydrous) 199329-67-4 (trihydrate)
Synonyms	β-D-Glucopyranosiduronic acid, 4-methyl-2-oxo-2H-1-benzopyran-7-yl, hydrate (1:x); 4-Methylumbelliferyl-β-D-Glucopyranosiduronic Acid hydrate; 4-Methyl-2-oxo-2H-1-benzopyran-7-yl-β-D-glucopyranosiduronic acid hydrate; 4-Methylumbelliferone glucuronide hydrate; 4-Methylumbelliferyl glucuronide hydrate; 4-Methylumbelliferyl β-D-glucuronic acid hydrate; Hymecromon-7-O-4-β-D-glucuronide hydrate
Purity	≥95%
IUPAC Name	(2S,3S,4S,5R,6S)-3,4,5-trihydroxy-6-(4-methyl-2-oxochromen-7-yl)oxyoxane-2-carboxylic acid;hydrate
Appearance	Solid Powder

Storage

Store at -20°C

4-Methylumbelliferyl-β-D-Glucuronide hydrate, a synthetic substrate used in biochemical assays to investigate enzyme activity, particularly β-glucuronidase, finds diverse applications:

Enzyme Activity Assays: Embedded within fluorescent assays to measure β-glucuronidase activity, 4-Methylumbelliferyl-β-D-Glucuronide hydrate undergoes hydrolysis by the enzyme, releasing 4-Methylumbelliferone, a fluorescent compound that simplifies quantification. These assays are pivotal in diagnostics and drug screening, providing insights into enzyme activity levels and potential therapeutic targets with precision.

Microbial Detection: In the realm of microbiology, this substrate aids in distinguishing bacterial species based on their β-glucuronidase activity, as it is incorporated into culture media to differentiate pathogens like E. coli through fluorescence production. This rapid identification tool ensures food safety and augments clinical diagnostic accuracy, offering a swift and dependable approach for microbial detection.

Cell and Tissue Studies: Researchers employ 4-Methylumbelliferyl-β-D-Glucuronide hydrate in cell and tissue investigations to monitor enzyme activity in situ, allowing for the observation of cellular processes and enzyme localization. The analysis of fluorescence distribution and intensity yields profound insights into disease pathology and cellular functions, aiding in comprehension of complex biological mechanisms and potential treatment strategies.

Biotechnological Applications: With its fluorescent properties, this substrate is utilized in the development of biosensors and biocatalysts for environmental monitoring and industrial processes. By enabling the creation of sensitive detection systems, it facilitates real-time monitoring of enzyme-catalyzed reactions, thereby enhancing process efficiency and control in biotechnological applications. This showcases its versatility and impact in biocatalysis and biosensor development.