4-Methylumbelliferyl-β-D-Glucuronide hydrate | CAS 881005-91-0

4-Methylumbelliferyl-β-D-Glucuronide hydrate, a synthetic substrate used in biochemical assays to investigate enzyme activity, particularly β-glucuronidase, finds diverse applications:

Enzyme Activity Assays: Embedded within fluorescent assays to measure β-glucuronidase activity, 4-Methylumbelliferyl-β-D-Glucuronide hydrate undergoes hydrolysis by the enzyme, releasing 4-Methylumbelliferone, a fluorescent compound that simplifies quantification. These assays are pivotal in diagnostics and drug screening, providing insights into enzyme activity levels and potential therapeutic targets with precision.

Microbial Detection: In the realm of microbiology, this substrate aids in distinguishing bacterial species based on their β-glucuronidase activity, as it is incorporated into culture media to differentiate pathogens like E. coli through fluorescence production. This rapid identification tool ensures food safety and augments clinical diagnostic accuracy, offering a swift and dependable approach for microbial detection.

Cell and Tissue Studies: Researchers employ 4-Methylumbelliferyl-β-D-Glucuronide hydrate in cell and tissue investigations to monitor enzyme activity in situ, allowing for the observation of cellular processes and enzyme localization. The analysis of fluorescence distribution and intensity yields profound insights into disease pathology and cellular functions, aiding in comprehension of complex biological mechanisms and potential treatment strategies.

Biotechnological Applications: With its fluorescent properties, this substrate is utilized in the development of biosensors and biocatalysts for environmental monitoring and industrial processes. By enabling the creation of sensitive detection systems, it facilitates real-time monitoring of enzyme-catalyzed reactions, thereby enhancing process efficiency and control in biotechnological applications. This showcases its versatility and impact in biocatalysis and biosensor development.