Inquiry Basket
Ac-DEVD-AFC | 201608-14-2
| Catalog Number | A18-0003 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C30H34F3N5O13 |
| Molecular Weight | 729.61 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A18-0003 | -- | $-- |
- Worldwide Delivery
- Quality Assurance
- 24/7 Customer Service
Product Introduction
Ac-DEVD-AFC is a fluorogenic substrate for caspase-3 and related caspases. Caspase activity can be quantified by fluorescent detection of free AFC (7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.
Chemical Information |
|
|---|---|
| Synonyms | Ac-DEVD-7-amido-4-trifluoroMethylcoumarin; Caspase-3 Substrate Ⅶ, Fluorogenic; Ac-Asp-Glu-Val-Asp-AFC; N-acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-α-asparagine |
| Purity | ≥90% |
| IUPAC Name | (4S)-4-[[(2S)-2-acetamido-3-carboxypropanoyl]amino]-5-[[(2S)-1-[[(2S)-3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-5-oxopentanoic acid |
| Canonical SMILES | CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C(CCC(=O)O)NC(=O)C(CC(=O)O)NC(=O)C |
| InChI | InChI=1S/C30H34F3N5O13/c1-12(2)25(38-26(47)17(6-7-21(40)41)36-28(49)18(10-22(42)43)34-13(3)39)29(50)37-19(11-23(44)45)27(48)35-14-4-5-15-16(30(31,32)33)9-24(46)51-20(15)8-14/h4-5,8-9,12,17-19,25H,6-7,10-11H2,1-3H3,(H,34,39)(H,35,48)(H,36,49)(H,37,50)(H,38,47)(H,40,41)(H,42,43)(H,44,45)/t17-,18-,19-,25-/m0/s1 |
| InChI Key | GZDRODOYEFEHGG-NUDCOPPTSA-N |
| Solubility | Soluble in Water |
| Density | 1.495±0.06 g/cm3 (Predicted) |
| Appearance | White Powder |
| Boiling Point | 1152.7±65.0°C (Predicted) |
- Product Specification
- Application
| Excitation | 400 nm |
| Emission | 505 nm |
| Storage | Store at -20°C |
Ac-DEVD-AFC is primarily utilized in apoptosis research, specifically for detecting caspase activity. Caspases, a family of cysteine proteases, play a critical role in programmed cell death. The substrate Ac-DEVD-AFC is designed to be selectively cleaved by caspase-3, one of the key effectors in the apoptosis pathway. By incorporating a fluorophore, this substrate enables researchers to monitor caspase-3 activity in real-time through fluorescence. This application is crucial for understanding the mechanisms of apoptosis and can aid in the development of therapies targeting cancer and neurodegenerative diseases, where dysregulation of apoptosis is a significant concern.
Another important application of Ac-DEVD-AFC is in drug discovery and development. By measuring the activity of caspase-3 in various cell lines treated with potential drug candidates, researchers can evaluate the pro-apoptotic effects of these compounds. This high-throughput screening method allows for the rapid identification of drugs that can induce apoptosis in cancer cells. Additionally, Ac-DEVD-AFC can be used to assess the efficacy of combination therapies, enhancing the understanding of drug interactions and synergistic effects in cancer treatment. This application underscores the substrate's role in advancing therapeutic strategies and improving patient outcomes.
Ac-DEVD-AFC is also instrumental in toxicology studies. It serves as a valuable tool for assessing the cytotoxic effects of environmental pollutants, chemicals, or pharmaceuticals on cellular systems. By measuring caspase-3 activity in cells exposed to these agents, researchers can determine the extent of apoptosis induced by toxic substances. This information is vital for regulatory assessments and risk evaluations, helping to establish safety profiles for various compounds. Furthermore, understanding the mechanisms of toxicity can inform the development of safer alternatives and improve environmental health.
Lastly, Ac-DEVD-AFC finds application in fundamental cell biology research, particularly in studies examining the regulation of apoptosis. Researchers can utilize this substrate to explore how various cellular signaling pathways influence caspase activation and activity. By manipulating factors such as growth factors, cytokines, and stressors, scientists can elucidate the intricate networks that govern cell survival and death. This knowledge is essential for developing strategies to manipulate apoptosis in various diseases, including cancer, autoimmune disorders, and degenerative diseases. The insights gained from these studies can lead to innovative therapeutic approaches that harness or inhibit apoptosis for clinical benefit.
Applications of Fluorescent Probes & Dyes
Get More Product Details?
